The Anti-tumour Effects And Its Mechanism Of The Aqueous Extract From Manchurian Walnut Bark

The Manchurian walnut bark is the bark of the carya known as the walnut family Juglans regia desiduous macrophanerophytes. It has been boiled for dringking to treat many tumours in civilian in our country. The chemical composition of the Manchurian walnut bark and its pharmacological action has been reported in many records, many compositions of which were the main component of the known anti-tumour drugs. its pharmacological action include dephlogisticate, analgesia, bacteriostasis, aldose reductase inhibition, anti-oxidation, biotoxicity, anti-tumour, and so on. However, there has not any report about the anti-tumour effects and its anti-tumour coponents of the manchurian walnut bark. The objective of this invistigation was to approach the anti-tumour effect of the aqueous extract from the manchurian walnut bark by observating its inhibition effect to the cultured tumour cell and the subcutaneous transplanted tumour of the BALb/c mice, to approach the anti-tumour coponents of the manchurian walnut bark and its mechanism of action by extracting and identifying the flavanoid from the aqueous extract and the effects to the p73 gene expression in the cultured cell of the flavanoid, thereby, providing foundation to the study of manchurian walnut bark becoming candidate anti-tumour drug composition. Result: 1. The aqueous extract had evident proliferation inhibition to the SMMC-7721 hepatoma carcinoma cell and the high dose of which has the effect to promote apoptosis. 2. The aqueous extract could evidently improve anaemia and immune state of the tumor-bearing mice and cut down its level of serum suppressor cytokine IL-10. 3. The main components of the flavanoid has been initially identified to be methoxyl flavone and naringenin. 4. The lower dose of the total flavanoid had effect to step up the level of p73 mRNA in the tumour cell and the higher dose cut down. Conclusion: The aqueous extract of the manchurian walnut bark has the effect to anti-tumour by inhibiting cell proliferation, promoting apoptosis and improving anaemia and immune state of the tumor-bearing mice. The main components of the flavanoid were some methoxyl flavone and naringenin. One mechanism of the anti-tumour effect of the flavanoid maybe implement by influnting the expression level of the p73 gene.