The Research On The Se-enriched Astragalus Milk Vetch Water Extract Of Anti-tumor And Mechanism

Objective The experimental study on Se-enriched Astragalus Milk vetch water extract(Se-AMWE) content detection with microwave digestion ICP-MS, drugcontaining serum on cell SGC-7901 proliferation in vitro and the effect of Se-AMWE on mice with H22 ascites and S180 solid tumor model mice in vivo, and discuss its antitumor mechanism of redox micro-environment. Evaluate the effects of Se-AMWE for possibilities tumor treatment, in order to develop new anti-cancer or auxiliary antineoplastic agents provide reasonable, theoretical and methodological Foundation of safe and effective.Methods 1、Se-AMWE mixed in different proportions extraction, and used microwave digestion-inductively coupled plasma mass spectrometry(Inductively coupled plasma mass spectrometry, ICP-MS) detection of Se content of selenium;2、SGC-7901 cells expose to blank serum and serum containing 24 h and then under a microscope for cell morphology; SGC-7901 cell adhesion after swapping with a 10% containing serum 24 h, 48 h and 72 h.Respectively, trypan blue staining of Se-AMWE containing serum on SGC-7901 cells in the blue dye uptake and MTT assay for detection of Se-AMWE containing serum inhibition of proliferation in SGC-7901 cell.3、Build mice H22 ascites tumor model and S180 entity tumor model, and each model mice random points into 8 Groups: model group(capacity saline)、 positive control group cytoxan(cyclophosphamide, CTX)(60mg/kg)、positive control group Selenium yeast(26μg/kg)、Astragalus extraction group(3.9g/kg)、Milk vetch extraction group(3.9g/kg) and Se-AMWE low、middle and high dose group(1.95g/kg, 3.9g/kg, and 7.8g/kg) per set of 12. Another 12 KM mice for the control group(such as volume of normal saline).After the last administration, H22 ascites tumor model experiment: All experimental groups stop the medication, and observe the effects of Se-AMWE influence on H22 mice with tumors of survival time. S180 solid tumor model experiment: 3.5% chloralhydrate anesthetized mice, take the tumor pancreas, spleen, thymus gland. Observe the effects of Se-AMWE on S180 solid tumor mice of inhibitory rate, immune organs index of body mass, abdominal aorta for blood, serum, detected the serum about seleniumbinding protein 1(SBP1), Glutathione peroxidase(GPX1), adenosine monophosphateactivated protein kinase(AMPK), reactive oxygen species(ROS) content by enzymelinked immunosorbent assay(ELISA).Results 1、After microwave digestion instrument procedures digestion Se-AMWE and inductively coupled plasma-mass spectrometry, selenium content is detected and compatibility of astragalus and Astragalus 1:1 extract of selenium content in the highest, with an average of 9.35 μg/ml.2、 Training SGC-7901 cell 24 h with10% containing drug serum can obviously change cell growth form; after cell normal training 24 h, instead each drug containing drug serum continues to training 24 h, 48 h and 72 h, and blank control group compared, SeAMWE containing drug serum group cell blue dye rate changes not obviously; MTT detection found Se-AMWE can obviously inhibit reduced OD value, and inhibit cell proliferation.3、 Respectively build the ascites tumor model in mice with H22 cells and entities tumor models of tumor S180 cell, for 10 days treatment. The H22 ascites tumor in mice: compared with the model groups, each group H22 prolonged survival of mouse ascites tumors. S180 entity tumor model mice: compare with model group, each drug group can obviously inhibit tumor organization growth, body quality no appeared obviously declined, immune organ index get improved; compare with model group, each to drug group SBP1 protein expression increased, GPX l activity improve, body organic Se content increased; and compare with model group, each to drug group improve AMPK level, and reduced ROS content.Conclusion 1、The highest selenium contents is obtained in the extract for mixture extraction of astragalus and Astragalus in accordance with 1:1 ratio.2、Se-AMWE inhibit SGC-7901 cell proliferation in vitro.3、Se-AMWE extending the survival time of H22 tumor-bearing mice. Se-AMWE inhibit the proliferation of the S180 tumor cells in vivo, increase the thymus gland, pancreas, spleen and other organs of the immune system Index. Each dose group could significantly increase the SBP1 protein expression in S180 mice, increased GPXl activity, increase AMPK levels, reduce the content of ROS, which may be associated with elevated selenium levels in tumor cells, effects of anti-cancer change tumor cell energy metabolism and micro environment.