| Objective: Successfully prepared mouse model of chronic bronchitis, lung andstomach on the basis of the theory of Chinese medicine, drawing on modern animalexperiments related technologies, through the model mice general, changes in lungtissue and serum pepsinate concentration level, etc. Comprehensive analysis, and thenfrom the perspective of experiments to validate the efficacy of cough particles of amouse model of chronic bronchitis, cough particles to investigate the effects of gastricfunction in mouse models, provides a basis for further study in laboratory medicinetheory.Methods: Choose healthy4-week-old Kunming mice SPF level42, male and female,weight (20±2) g, male and female sub-cage adaptive feeding1week. Then usingdivided into normal group10,32model group, model group smoked modeling method,randomly from depicting a male and female mice smoked four weeks after lung biopsyobserve pathological changes consistent with chronic bronchitis prompt successfulmodeling; Grouped again according to the model group30mice were divided intountreated group10, pediatric lung cough particle group10, group10cough particles,and then fed intervention, the normal group and model group were given the samevolume the saline gavage,pediatric lung cough cough particles were given particlesuspension pediatric pulmonary gavages, cough cough particle suspension particleswere given orally. Be administered1week after drawn, The mice eyeball blood,serum concentrations of pepsinate; Take the middle part of the right lung biopsy oflung tissue of mice was observed.Results: Normal mice without cough, steady weight gain, eating normal water,freedom of movement, hair glowing skin, normal stool; In modeling the model miceafter1week, occasionally coughing, scratching nose etc, gray hair yellow, gloss poor,huddled mouse easy to get together, slow weight gain, eating too few, stool thin paste, smoked4weeks after the above are symptoms, until the intervention of varioussymptoms were not relieved; Pediatric lung cough particles after intervention groupwere cough medicines to reduce body weight increased slightly compared with themodel group, the hair has improved, eating in general, the stool is soft stools; Aftercough particles similar set of symptoms in mice with the model group smoked,occasionally coughing after drug intervention, grasping nose movements, rapid weightgain, eating well water, hair shiny, slightly dark, normal stool. Lung biopsy showed:normal mice end bronchial clear texture, structural integrity, non-epithelial cellnecrosis, no inflammatory cells, serous exudate;Bronchial lumen model mice haveserous, inflammatory exudates cells, some structural damage to the bronchial wall,most of the epithelial cell necrosis, or even fall off, the surrounding tissue of the smallvein dilation, blood swelling significantly, goblet cell hyperplasia; Cough particleswithin the group and the pediatric lung particles have a small group of lung tissueinflammatory cell infiltration, the structural integrity of the wall, texture is slightlyrough, the surrounding tissue can be scattered small veins mild congestion andswelling. Serum PG levels: normal group, model group compared with the serumpepsinate concentration decreased (P<0.05); Compared with the model group, thegroup of pediatric pulmonary cough particles serum pepsinate concentration increased(P<0.05); Compared with the model group cough particles in serum pepsinateconcentration increased (P<0.05); Cough particles in serum pepsinate pediatric lungcough particles were higher than group (P<0.05).Conclusion: Weakened stomach function in mice with chronic presence in the course;Cough chronic inflammation of the lungs of the particles has a therapeutic effect, butcan enhance the function of the stomach; Efficacy in treating chronic bronchitis coughparticles than pediatric lung cough particles. |
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