Compound Yunnan Dengzhanhua Capsules Effect In Chronic Renal Failure Smad7, CTGF Factor Signal Expression Research

Objective:This topic proposed by animal experiment in rats, from the level ofmolecular biology research Compound Yunnan Dengzhanhua capsules on5/6nephrectomy chronic renal failure rat kidney Smad7, the signal of CTGF expression,discusses Compound Yunnan Dengzhanhua capsules on prevention and control anddelay the mechanism of chronic renal failure. With advanced modern molecularbiology research means, provide scientific theory basis for drug efficacy.Methods:Select healthy male SD rats60, only about180grams, domesticationfeeding A week later, A random sample of15as normal control group (group A), onlythe remaining45only as A treatment, platt5/6renal excision method productionmodel of chronic renal failure. Experimental animal breeding to2weekspostoperatively, all blood, urine and serum urea nitrogen (BUN), creatinine (Cr) and24h urine protein. Scr and BUN of rats is significantly higher than normal group forbuilding a successful. Creatinine values based on average are divided into3groups(each group of15only): the model group (group B), lamp group (group C), los DingXin group (group D). Rats for12consecutive weeks after death, return blood andkidney tissue samples. Detection of serum BUN, Scr; Rt-pcr detection of renal tissueCTGF expression, Western Blot method to detect renal Smad7protein expression ofsignal within the organization.Results:(1) the change of the Scr, BUN, model group, lamps, Scr, BUN, value oflos Ding Xin group were significantly higher than that of normal group, model group,the highest Ding Xin group is significantly higher than lamps flower group, withstatistical significance (P <0.05).(2) the CTGF, the change of Smad7: model group,lamps, CTGF value Ding Xin group was obviously higher than that of normal group, model group, the highest Ding Xin group is significantly higher than lamps flowers,with statistical significance (P <0.05);(3) the model group, lamps, los Smad7mRNAexpress Ding Xin group was obviously lower than normal group, model group, thelowest Ding Xin significantly below lamp flower group, with statistical significance(P <0.05); Lamps flower capsules can delay the progress of chronic renal failure.Conclusions:Compound Yunnan Dengzhanhua capsules5/6nephrectomy rat kidneytissues by raising the expression of Smad7, inhibit the expression of CTGF, tointervene in the renal interstitial fibrosis, slow progress in rats with renal failure, itscurative effect is better than that of hydrochloric acid benzene that split.