The Study Of The Character Of Genovariation Of CFH Gene In The Age-related Macular Degeneration Of Liver-kidney Yin Deficiency Type

BackgroundAge related macular degeneration(age-releated macular degeneration,in the developed country Home Furnishing allcause of blindness degeneration, in the developed country Home Furnishing allcause of blindness in the first,in recent years, with Chinese aging population, the incidence of AMD is rapidly rising, gradually become the focus of blinding eye diseaseincidence rate in Changning, only Shanghai from200317.7%to200829.9%, accounted for21.5%of new blind, beyond the reversible blind cataract blindness, promoted Shanghai first. Bring heavy economic burden to patients and their families and the society, it will be AMD listed as one of the key topics of the Department of Ophthalmology researchto prevent blindness. The pathogenesis of AMD is unclear, the study of the pathogenesis of AMD has been the hot topic in the field of Department of Ophthalmology, oxidative stress theory, motivation theory, blood rod cells susceptible theory research for many years, has a guiding role to the clinical treatment, advances in genetic theory and inflammatory immune theory research rapidly in recent years, has been found many AMD related genes, such as complement factor H (com-plement Factor H, CFH), complement factor B (CFB)gene, Complement2(C2) gene,VEGF gene, LOC387715gene, CX3CR1gene, apolipoprotein E (APOE) gene,ABCA4, ABCI, VLDLR, HEMICENTIN1gene and LRP6etc. there is some relation with the pathophysiology of AMD. The CFH has been generally recognized, the mutation of CFH gene is the key factors of AMD disease, is also considered to be a great success of AMD gene obtained. So that this research project, the mutation of CFH gene in the gene polymorphism is a key pathogenic AMD. Because of the wide use of Chinese medicine clinical, pace but fuzzy mechanism syndrome connotation and drug problems AMD Chinese medicine research, it is necessary to learn from the new modern medical pathogenesis studies, contact the bridge between the pathogenesis and syndromes of traditional Chinese medicine AMD construction, and based on the treatment of entry point AMD with traditional Chinese medicine.PurposeBy using the method of molecular biology, comparative study was conducted on30cases of liver kidney yin deficiency AMD patients and30normal people, to investigate the relationship between the liver and kidney yin deficiency type age-related macular degeneration, the mutation of CFH gene and the pathogenesis of AMD, to further explore the C allele is liver kidney yin deficiency type AMD disease causing high risk factors. And to provide the scientific basis for the development of clinical prevention of age-related macular degeneration.Research MethodThis project uses the prospective research trials, in2012January-2014year in March period in the Department of Ophthalmology of Guangdong Province Traditional Chinese Medical Hospital outpatient and inpatient treatment by conventional AMD examination and clinical safetyscreening,30patients met the inclusion criteria of yin deficiency of liver and kidney in24cases of AMD patients, dry, wet6cases,10males,20females in30cases of normal people, among them,18cases of male,12cases female, acquisition of AMD patients and normal human peripheral blood, DNA extraction, PCR amplification and sequencing, detection of CFH gene polymorphism, were analyzed using SPSS18.0statistical software, analysis of CFH gene polymorphism between genotypes forcount data, compared with χ2inspection, P<0.05has statistical significance, yin deficiency of liver and kidney of CFH patients with AMD gene mutation, to further explore the C allele is causing the high risk factors of liver kidney yin deficiency type AMD.Results1.30cases of liver kidney yin deficiency type AMD,3was type TT,10%,6was type TC,20%,21was type CC,70%,19was type TT,30cases of normal people accounted for63.3%,8was type TC,26.7%,3was type CC,10%, after statistical analysis, AMD patients and normal people frequency genotype polymorphism of CFH gene had significant difference (χ2=25.42, P=0.00<0.05). 2. The Callele in AMD patients accounted for80%, accounting for20%in normal subjects, the allele T in AMD patients accounted for76.7%, accounting for23.3%in normal people, there is significant difference between AMD patients and normal people frequency distribution of T/C (^=38.58, P=.00<0.05).3. Yin deficiency of liver and kidney of AMD patients and normal people age with normal distribution, the results of t test show that.no statistical significance between the age between AMD patients and normal people (t=0.06,P=0.95), through the use of χ2draws, no statistical significance between the sexes (χ2=0.29, P>0.05).Gonclusion1. liver kidney yin deficiency type AMD and CFH polymorphisms of Y402H gene TC/CC allele and genotype C, genotype CFH showed regular distribution in the liver and kidney yin deficiency type AMD patients in the CFH group, the homozygote CC or/and major gene heterozygote TC is liver kidney yin deficiency type AMD type. The C allele was the high risk factors causing liver kidney yin deficiency type wet AMD.