| Objective:This study aims to study the anti-diabetic effect and working mechanism of the classical formula of Treatise on Febrile Diseases. Through the adoption of animal experiments with the basic theory of TCM and modern medical technology, the author observes how Huanglian Ejiao decoction of modified dosage can affect GLU, TG, CHOL, FIN, HOMA-IR, LP of diabetic rats. By monitoring the changes of those above mentioned index and their significance in treating diabetes, this paper attempts to assess the anti-diabetic effects of Huanglian Ejiao decoction of modified dosage and its working mechanism. To conclude, this thesis will contribute to the breath and depth of studies on anti-diabetic TCM compound preparations, thus providing an effective platform for the assessment and promotion of clinical use of TCM in treating diabetes.Methods:To begin with, this paper discusses the pathogenesis of diabetes in the views of TCM and western medicine as well as its treatment mechanism in these two fields respectively. Secondly, after a general literature review of the clinical use of and research progress on Huanglian Ejiao decoction, this paper summarizes the knowledge of Huanglian Ejiao decoction by medical professionals throughout history. On this basis, the author proposes the basis of this research:the principle of Ku Suan Zhi Tian。In this research,50SFP level SD rats are selected, of which25are male and the other25female. Then they are randomly divided into4groups, namely treated, gliclazide, model, blank control, with14in each of the first three groups and8in blank control group. Rats in treated, gliclazide and model groups are given intraperitoneal injection of STZ with the dose of55mg/kg. When models are done, treated group and gliclazide group are given by gavage with the dose of30g/kg and20mg/kg respectively, while blank control and model groups are given the same amount of distilled water, all of which are given once a day and last for2weeks. In research period, each group is given normal feed. After2weeks of gavage, the rats are anesthetized, taken blood sample and killed. The first research studies the affect of Huanglian Ejiao decoction of modified dosage on blood sugar level of diabetic rats. In order to assess whether the model has been set successfully, rat blood is taken from the cut tails to test their random blood sugar level. In this step, the test is done with minute glucose monitors. Then, after the anti-diabetic treatment has been done, the level of fasting glucose is tested with automatic biochemical analyzer using blood taken from aorta abdominalis of rats. The second test studies affect of modification of dosage of Huanglian Ejiao decoction of modified dosage on TG, CHOL, FIN, HOMA-IR, LP of diabetic rats. With blood taken from aorta abdominalis as the test sample, the level of TG and CHOL is analyzed by automatic biochemical analyzer, while the level of FIN and LP is analyzed with RayBio kit. Here insulin resistance index is calculated with the following formula:HOMA-IR=FPG (mmol/L)×FINS(mU/L)/22.5. This research takes the natural logarithm as a result.Results:1. Compared with the blank control group, fasting glucose level is generally higher in treated, gliclazide and model groups rats that have been treated with Huanglian Ejiao decoction of modified dosage. Specifically, in terms of fasting glucose level, the difference between blank control group and treated group as well as model group is statistically significant (p<0.05), while that between gliclazide group and blank control group is not(p>0.05). Meanwhile, there is statistically significant difference between fasting glucose level of gliclazide group and blank control group (p<0.05), while no significant difference has been found in that between treated group and model group. Nevertheless, the mean difference between treated group and model group is about-1.2. No statistical significance has been found in the difference of all the tested index among different rat groups.Conclusion: Gliclazide is effective in reducing blood sugar level. Though no statistically significant difference has been found in fasting glucose level between treated group and model group, their mean difference stands at about-1. According to the observation of the author, rats of treated group shows less diabetic symptoms than those in model grou and perform better after treatment. Therefore, it is reasonable to assume that TCM exerts certain anti-diabetic effect. Several elements may contribute to the lack of strong anti-diabetic effect of TCM:the short duration of treatment, the limited number of sample, lack of correspondence between TCM prescriptions and symptoms as well as imperfection in pharmaceutics and method of feeding medicines. Meanwhile, in terms of TG, CHOL, FIN, HOMA-IR and LP, there is no significant difference among different rat groups. Since such lack of difference is presumably due to the fact that the models that was built does not induce obvious insular resistance, this paper is unable to discuss the relevance between the anti-diabetic mechanism of Huanglian Ejiao decoction of modified dosage and insular resistance. |
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