Gabexate Made NF-κB Impact On Research In The Rat Hepatic Ischemia-reperfusion Injury

【Objective】Before establishment of SD rat liver warm ischemia-reperfusion injurymodel,application of Gabexate on these rats preemperimentally to investigate thethermal effects of ischemia and reperfusion in rat liver in NF-Kb and use this toprovide theoretical basis for clinical studies to reduce liver gabexateischemia-reperfusion injury.【methods】1.Use Pringle’s method to establish pan-liver hepatic ischemia-reperfusion models.2. SPF grade72healthy rats, male or female, weighing between250g-280g, dividedthem into four groups randomly: Group A (surgery group) were just dissected theliver duodenum ligament after finish laparotomy; Group B (ischemia-reperfusiongroup),Hepatic portal structures exposed at this group after finish laparotomy,clamping the all the tissue of the Hepatic portal structures, made them into all of thehepatic ischemia,45minutes underwent reperfusion; group C (gabexate treatmentgroup) in patients2hours ago, intraoperative give gabexate solution, with theremaining treatment group B; D group (saline control group) with a dose of salinesolution to replace gabexate, waste treatment methods in the same group C. Eachexperiment object again divided into three subgroups (n=6), each subgroupischemic times are all45minutes but reperfusion time are varies: T1time pointsubgroup reperfusion1hour, T2time points subgroup2h of reperfusion, T3timepoints subgroup reperfusion3hours.3measured indicators: In the set phase point rats were killed, exsanguinate5mlvenous blood from inferior vena cava, clipping part of the liver tissue, serum ALT,AST, plasma SOD, TNF-α﹑IL-8, MDA ’s activity and liver cell morphology were observed line NF-Kb immunohistochemistry to observe the changes.【Results】1successfully established72rats model of hepatic ischemia-reperfusion injury,all therat,s hepatic portal structures were blocked for45minutes.2. Comparison among4groups rats of ALT, AST content in serum is as follows:When the same time point four groups of rats comparison; B, D two groups wereno significant difference (P>0.05) and they are the most obvious indicator rises,group A is the less one in all groups, group C more than group A, less than B, Dgroups (P <0.05), the sublinguals in the same group comparison: group A nosignificant change (P>0.05), B, C, D groups from T1to T3time points tended toincrease (P <0.05).3Comparison4groups rats of SOD content in plasmaAt the same point comparison between the four groups of rats group; B, D groupsignificant difference (P>0.05) and the lowest content of SOD, highest in group Aand group C lower than A, higher than the B, D group (P <0.05), the sublinguals inthe same group comparison: groupA no significant change (P>0.05), B, C, D threegroups T1to T3time points showed a decreasing trend (P <0.05)4. Compare4groups plasma TNF-α, IL-8, MDA contentAt the same time point comparison between the four groups of rats; between Band D were no significant difference (P>0.05) and the higstest level in thoseindacators, the lowest in group A, group C more than group A, less than B, D groups(P <0.05), the sublinguals in the same group comparison:groupA have no significantchange, B, C, D three groups from T1to T3showed a decreasing trend (P <0.05)5. Comparison the4groups of HE staining about liver tissue in the set time points.GroupA of various time points lobular structure rules, periportal hepatocytes andnormal structure.Group B the liver cells have different levels of change, fattydegeneration of the liver sinusoid congestion, punctate necrosis,T1to T3significantlyincreased significantly. Group C liver cell edema, partially visible steatosis, showing only a small amount of inflammatory cells, T1to T3, the cells were injuriedhardly and little vision could see spotty necrosis, no massive cell necrosis, whilelighter points compared with group B, D and B group groups like.6Changes in NF-Kb liver tissueA group at each time point liver cells almost have shown no activation ofNF-Kb; As for Group B varying degrees of NF-KbActivation phase point T1isonly found in the cytoplasm when the orange change,T2time points changes in livercells occurs orange range increases, some cells appear orange nucleus and some otherchanges when the phase change point T3, large amount of cytoplasm of hepatocytesshow orange change, nucleus show brow colored change. Compare with GroupCand D, cell injury is lighter and nuclei dark brown large-scale change does notappear.The observed results of Group D are similar to those of group B.