| Objective: Through building the rat intestinal ischemia reperfusion injury model, theauthor measures the level of XOD, MDA, TNF-α, IL-8in blood. And the authorobserve the liver and intestinal in pathologic histology. Then by applying the model ofGabexate Mesilate pretreatment,the author discusses the function and the mechanismof gabexate mesilate on intestinal ischemia reperfusion liver, intestinal injury.Method:Firstly, divide randomly the Sprague Dawley (SD) male rats45, weightrange of220-300g (provided by the animal experimental center of Kunming MedicalUniversity), into A, B, C three groups. Each group is subdivided into T1, T2, T3group, group T1is the30min group, group T2is the60min group, group T3is the120min group. Secondly, group A is the sham operation group (Sham group), freesthe superior mesenteric artery without any treatment. After30min,60min,120min,puts the rats to death and gets the inferior vena cava blood. At the same time, clips thetissue from the ileocecal3cm intestinal and left lateral lobe of liver tissue. Thirdly,group B, the ischemia reperfusion and gabexate mesilate preconditioning group(group GM), are injected by the sublingual intravenous (10mg/kg.h), after30minminutes, makes a2cm cut at median abdominal, frees the superior mesenteric artery(SMA) and clips the SMA root with the lossless clamp to block the arterial blood.After60min, remove the artery clamp and reperfusion. After30min,60min,120min,immediately open the abdomen to get the samples. The group C, the ischemiareperfusion group (group IIRI), is injected the saline by preoperative sublingualintravenous the same dose of B group, the operation, materials are same to the groupB. measures the level of ALT, AST, XOD, MDA, TNF-α, IL-8content in the bloodsamples. The liver, intestinal specimens are detected pathologically. Result:1. The Changes of ALT, AST content in the serumIn T1, T2, T3period, group C is significantly higher than group B and A (P <0.05),group B is higher than group A (P <0.05). The ALT, AST increasing in the serum arepositively correlated with time of reperfusion. Group GM is effective in the treatmentof intervention.2. The changes of MDA content in blood plasmaAt the three time points, group A MDA run at a low level, and there are no obviouschange (P>0.05), group C begin to increase at time T1, reach the peak at time pointT3. Group B is between group A and group C, the value of group B increase with thereperfusion time. Compared with group C, there was significant difference in groupB(P <0.05).3. The changes of XOD activity in plasmaThe XOD of A group has no obvious change; group B reaches the peak at T2timepoint, and continue the peak value at T3point in time. Group C is same to group B inthe trend, but the content is higher than group B, the difference is statisticallysignificant (P <0.01).4. The changes of TNF-α, IL-8in plasmaThe TNF-α, IL-8C in group B and group c increase with the reperfusion time, andreached the peak at T3time point. At each time point group B is lower than group C,the difference was statistically significant (P <0.05). The TNF-α, IL-8in group Adon’t have any changes and relationships with the time.5. The pathological changes of intestinal, liver tissueThe test results of pathological section: group B, group C, Chiu score changes obviously with the reperfusion time, reached the peak at T3time point. Group B islower than that of C group at each time point. liver cell appears swelling in liver tissuein C group in T1and inflammatory cell infiltration, at T3time point, the liver cellschanges like balloon and partial liver cell apoptosis. At T2time point in group B, theliver appears pathological changes of sinus gap widened and cell swelling, the deathrate liver cell is still low at T3time point.Conclusion:1. the TNF-α,IL-8,free radicals can lead to the liver injury through blood circulation.2. Gabexate mesilate has protective effects on the liver and intestinal, possiblythrough inhibition of free radicals, TNF-α, IL-8, and reducing the lipid per-oxidationeffect, and stabilizing the biofilm and reducing inflammation cell apoptosis and tissuegeneration... |
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