The Effect And Mechanism Of Extractive Of Houttuynla Cordata Thunb On Mast Cell Activation

Objective:mast cells (mast cell) is widely distributed in the connective tissue of skin and visceral mucous membrane, has strong basophilic granular cells. Secretion of cytokines, involved in immune regulation, abundant expression of IgE recepto r Fc, the release of allergic media. Mast cells and substance particle release, co ntaining heparin, histamine,5-hydroxytryptamine, can cause anaphylaxis. The m ast cells obtained from SD rats abdominal cavity, observed mulberry (Cortex M ori) extract on Anti-DNP IgE induced mast cell degranulation, histamine release, intracellular calcium intake. To observe the effects of vascular permeability of white mulberry root bark extract on Anti-DNP induced by IgE in rat skin allerg ic reactions.Methods:Induction of rat skin allergic reaction by Anti-DNP IgE (PCA) experiment, using the Evans blue dye to observe the vascular permeability; degranulation o f mast cell induced by using Anti-DNP IgE, collected from rat peritoneal mast cells, and divided into five groups, normal group, model group, white mulberry root bark extract low concentration group (2mg/ml); concentration group of whit e mulberry root bark extract in (20mg/ml); white mulberry root bark extract of high concentration group (200mg/ml), by measuring the effect of Herba Houttuy niae on mast cell assay in vitro, white mulberry root bark extract on Anti-DNP IgE induced mast cell degranulation, histamine release, effect of intracellular ca lcium intake.Results:Mulberry bark extract with different concentration has no obvious effect on mast cells showed that the absorbance, white mulberry root bark extract on the survival of mast cells had no obvious effect. Anti-DNP IgE could induce mast cell degranulation, given60mg/ml and120mg/ml of Cortex Mori extract pretreatment, inhibit the degranulation of mast cells. The model group compared with normal group, histamine release from mast cells increased significantly (P<0.05), compared with the model group of white mulberry root bark extract groups were inhibited histamine release of mast cells, which, in (200,20mg/ml) compared with the dose group and model group, there was statistically significant (P<0.05). Evans blue extravasation rarely in normal group, model group compared with normal group of Evans blue extravasation was significantly increased (P<0.05), compared with the model group of white mulberry root bark extract groups can reduce the Evans blue exudation, including high, in (200,20mg/kg) compared with the dose group and model group, there was statistically significant (P<0.05). The normal group of mast cell and calcium intake less. Compared with the normal group, model group, mast cell calcium intake increased significantly (P<0.05), compared with the model group of white mulberry root bark extract groups were able to inhibit mast cell calcium intake, which is high,(200,20mg/ml) compared with the dose group and model group, there was statistically significant (P<0.05).Conclusion:Mulberry bark extract inhibited the activation by calcium influx of mast cells, inhibition of mast cell degranulation and histamine release, reducing vascular permeability of mast cell mediated.