The Effect And Mechanism Of Timosaponin On Mast Cell Activation

Objective:Saponins from Anemarrhena Asphodeloides Bunge (SAAB) containing of Notopterygium effective components, has obvious anti-inflammatory, antioxidant and other pharmacological effects. Although has been shown to extracts from Notopterygium has strong anti-inflammatory effect, but for the treatment of allergic asthma and its mechanism has not been reported. This experiment will investigates the effects of extracts from Inonotus obliquu on Th2cytokines secretion in murine asthma, and discuss about extracts from Notopterygium anti asthma effect and its possible mechanism.Methods:The experiment of passive cutaneous anaphylaxis (PCA) was used to determine the effect of SAAB on degranulation of mast cells in vivo. Rat peritoneal mast cells (RPMC) were culture in vitro. MPMC were divided into five groups, normal group, degranulation group, SAAB low concentration group (SAAB25); SAAB middle concentration group (SAAB50); SAAB high concentration group (100SAAB), For in vitro study, various doses of SAAB were added to sensitized rat peritoneal mast cells. The IgE-induced release of histamine and calcium intake was measured.Results:The concentration of SAAB in25～100μg/L had no significant effect on the absorbance of the mast cells. It suggested that SAAB had no obvious effects on the survival of mast cells. Mast cell degranulation was induced significantly by anti-DNP IgE. Mast cell degranulation was not inhibited by25μg/L SAAB pretreatment, but mast cell degranulation was not obvious and significantly inhibited by the pretreatment of SAAB in50or100μg/L. Histamine generation was very low in normal group, which was significantly increased in degranulation group compared with in normal group(P<0.05). Histamine generation was reduced by all of SAAB treatment group, which was statistically significant in high or middle concentration group (100,50μg/L) compared with in degranulation group (P<0.05). Evans blue exudation was little in normal group, which was significantly increased in model group compared with in normal group(P<0.05). The exudation of dye was reduced by all of SAAB treatment group, which was statistically significant in high or middle dose group (300,150mg/kg) compared with the model group (P<0.05). Calcium intake was little in normal group, which was significantly increased in degranulation group compared with in normal group(P<0.05). Calcium intake was reduced by all of SAAB treatment group, which was statistically significant in high or middle concentration group (100,50μg/L) compared with the degranulation group (P<0.05).Conclusion:(1) Mast cells degranulation and histamine release was significantly inhibited with SAAB treatment in rats.(2) plasma leakage mediated by mast cells was significantly inhibited with SAAB treatment in rats.(3) Mast cells degranulation was significantly inhibited with SAAB treatment in rats through calcium influx decrease in mast cells...