| Objective:1. To investigate the correlation of the amplification of hTERC gene and c-myc gene detected by mFISH in CIN and cervical cancer and the cervical lesion level.2. To discuss the influence of the amplification of hTERC gene and c-myc gene to the prognosis of cervical lesion.3. To discuss the relationship between the amplification of hTERC gene and c-mycgene,cervical cancer clinicalstage,pathological grading,lymph node metastasis.Methods:150cases with cervical scc and CIN patients diagnosed by pathology as reseearch subjects were selected from the Affiliated Hospital of Jining Medical University from January2007to January2009. Wax samples of the cervical lesion were kept well, clinical data were complete. These cases were divided into four groups:30cases of CIN I grade,30cases of CIN Ⅱ grade,30cases of CINIII grade, and60cases of patients with squamous cervical cancer. The ages were from23-66, and average age was38.8.34cases of G1,17of G2,9of G3,37of FIGO stage I,23of FIGO stage Iia;13cases with lymph node metastases, and47without metastasis. We selected20women with normal cervical as a control group. We detected the expression of hTERC gene and c-myc gene in the170cases selected by mFISH. These patients were followed up for five years.Results:1. The positive amplification rate of hTERC gene, c-myc gene gradually increased respectively in all the five groups. Positive rates of hTERC and c-myc were5.0%,23.3%,63.33%,90.00%and5.00%,13.33%,53.33%,86.67%,90.00%, respectively. There was no statistically significant difference the two genes of the positive amplification rate in various pathological groups (P>0.05).2. The amplification of hTERC vs. c-myc gene in each group:(1) There was statistically significant difference in hTERC gene amplification between CINI and CINII, CINIII and cervical cancer group, between CINII and CINIII, cervical cancer group (P<0.007). There was no statistically significant difference in the comparison of other groups.(P>0.007). (2) C-myc gene positive amplification rate was higher in CINII and over lesions than it in CINI and normal group (P<0.007). CINII compared with CINIII and cervical cancer group, the differences were statistically significant (P<0.007). Normal group compared with CINI and CINIII compared with cervical cancer group, there was no statistically significant difference (P>0.007).3. The amplification of hTERC gene and c-myc gene at the same time is an important expansion type. Only hTERC gene amplified in few cases. C-myc gene amplification was not detected alone. There were5%cervical cancer patients that we did not detected the hTERC gene and c-myc gene amplification. Only hTERC gene amplification was detected in5%cervical cancer patients.4. There was no statistically significant difference of the positive amplification rate of hTERC gene and c-myc between different clinical stage, different pathologic grading and Lymph node metastases of cervical cancer (P>0.05).5. All relapse cases in five years were detected in hTERC and c-myc gene expansion.2/4patients with both hTERC and c-myc gene amplification evolved in CINI group. Compared with CINII and CINIII, cervical cancer group, recurrence rate was significant difference (P<0.05). The hTERC, c-myc gene amplification rate have nothing to do with5-year survival rate of cervical cancer.Conclusion:1. The positive rate of hTERC and c-myc increased with grades of cervical lesions.2. hTERC and c-myc gene can can predict cervical lesions progress. They might be important auxiliary biological indicators in pathological detection of cervical low-grade or highly lesions.3.Compared with c-myc,the amplification of hTERC gene maybe play a more important role in the process of the occurrence of cervical squamous cell carcinoma.4.With analysis of colposcope and HPV examination,active treatment shoud be adopted in CINI cases which hTERC, c-myc gene amplification. hTERC gene, c-myc gene amplification rate have nothing to do with5-year survival rate of cervical SCC. |
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