| Objective:Intracranial pressure process of this study was to compare the quick establishment of pig model of brain death and, where drug-free pigs under brain death and non-superoxide dismutase in serum of brain death (SOD), malondialdehyde (MDA), Interleukin-6(IL-6), monocyte chemotactic protein1(MCP-1) and other content changes. Myocardial biopsy superoxide dismutase (SOD) mRNA expression. Changes of myocardial pathologic observation of myocardial cells, heart rate, EEG and intracranial pressure and mean blood pressure assessed, and oxygen free radical in patients with acute cerebral death pig brain death affects the heart donor.Method:6head of Xishuangbanna miniature pig health were randomly divided into2groups (n=3) and the experimental group (group a) and control group (Group b). Experiment group vein body anesthesia down tracheal cut intubation, breathing machine auxiliary breathing, neck within moving vein intubation, and in right skull vector shaped line outside side0.2-0.5cm Department and left skull vector shaped line and Crown loaded line junction Department of outside Shang two-thirds, distance vector shaped line0.2cm Department the drill a diameter about lcm of hole, a root Foleyl8F airbag catheter tube by right hole reset into hard Meningioma Xia cavity within, to right airbag catheter tube in the to2ml/s injected25-40ml saline, gradually expansion airbag, Increased intracranial pressure and injection time in1minute, Foley pressure balloon catheter to establish models of acute brain death and maintain10h, left the wires along the drilling into the subdural intracranial pressure sensing Chamber, recording and observation of changes of intracranial pressure before and after brain death. Controlled intravenous general anesthesia and tracheal intubation, internal jugular vein intubation after0.9%saline and anaesthetic drugs to maintain. After the experimental group in the establishment of model of acute brain death, dopamine in saline and maintaining a CVP-5-10cmH2O,MAP0.9%to60mmHg-90mmHg. If Diabetes Insipidus of antidiuretic hormone, dose, time and amount of urine to keep detailed records. Experiments during the experimental group and the control group are the same, but only the experimental group to establish models of acute brain death. Apply a quick model of intracranial pressure method for establishing brain death through respiratory and circulatory support maintenance of laboratory animal brain death10hours,10hours after the removal of respiratory and circulatory support. A b two groups of experimental animals, respectively0.5h,2H,4H,6h,8h,10h, take arterial blood6ml detection in serum superoxide dismutase, malondialdehyde, Interleukin6, monocyte chemotactic protein1content. In brain death-0.5h,2H,4H,6h,8h,10h, measuring heart rate, ECG, EEG, mean arterial pressure, experimental extraction of left ventricle tissue about2blocks in the late1.0g to hold alternate.Results:1. Before the adoption of intracranial pressure rapidly established in pigs brain-dead model,6pigs,100%surgery, postoperative survival rate of10h model90%, died during the model1,5pigs for research. Failure of MF in the experiment died of ventricular fibrillation.2:Hemodynamic and cardiac medication dosage:intracranial pressure in experimental group by quickly established pigs brain-dead model, later with the gradual decline of cardiac function, mean arterial pressure (MAP) decrease central venous pressure (CVP) rise, low blood pressure, need for vasoactive drugs dopamine to maintain basic blood pressure. Experimental group in pigs brain-dead model is set up, the dosage of dopamine than the control group increased significantly, brain death heart rate controlled significantly slowed down.3:Change of SOD activity in serum:increased serum SOD levels in the experimental group than in the control group. Changes of serum SOD levels in the experimental group and the control group with significant differences in the0.5h,6h8h (P<0.05). 4:Change of MDA content in serum:elevated serum MDA content in the experimental group compared with the control group. Content changes of serum MDA in the experimental group and the control group at8h、10h had significant difference (P<0.05).5:Changes of serum IL-6activity:the experimental group and the control group in10h, higher than in the control group. Changes of serum IL-6activity in the experimental group and the control group had significant difference (P<0.05).6:Changes of serum MCP-1activity:the experimental group and the control group in10h, higher than in the control group. Two group MCP-1exists significantly differences (P<0.05).7:Myocardial SODmRNA expression:experiment group SOD1and SOD2obviously above controlled group, experiment group and controlled group SODmRNA expression has significantly differences (P<0.05).8:Under optical microscope observation of myocardial tissue:experimental myocardial interstitial edema, some granular degeneration myocardial fibrosis, interstitial vascular contraction, samples of muscle fiber degeneration, inflammatory cell infiltration, and muscle fibers rupture. Neat and myocardial fibrosis in the control group, horizontal stripes clear. Experimental and control groups with significant differences.Conclusions:1. The application of intracranial pressure rapidly established pigs brain-dead model, more in line with the process of clinical development of brain death, effective respiratory and circulatory support after acute brain death may remain stable.2. The decline in cardiac function is an important pathophysiological characteristics of heart after acute brain death, myocardial injury in pigs can lead to brain death, and myocardial cell morphology changes.3.Using EEG and detection can increase intracranial pressure in patients with acute brain death judgment accuracy.4Generation of MDA and IL-6MCP-1after acute brain death increased SOD capable of scavenging oxygen free radicals and reducing biofilm formation of lipid peroxidation and inflammatory factors5Heart SODmRNA high degree of expression of cardiac muscle during acute cerebral death, indicate themselves under brain death status in acute myocardial antioxidant function. |
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