Relationship Between CyclinD1Expression And Clinicopathological Characteristics In Non-small Cell Lung Cancer

Objective: To observe and explore the expressions of cyclinD1and p16innon-small cell lung cancer and the relationships and significances between theexpressions and the pathological characteristics of non-small cell lung cancer.Methods: Specimens(archival paraffin blocks) were collected from2012-2014inthe first affiliated hospital of Dalian Medical University. Expression of CyclinD1andP16in143cases of NSCLCs and37cases of pulmonary tissues from inflammatorypseudotumors were detected by maxvision immunohistochemical method. All patientshad no history of radiation and chemotherapy or other anti-tumor treatment beforeoperation. Chi-Square test and Spearman correlation test were used to analyze the datawith SPSS20.0software package.Results:1. The overexpression rate of CyclinD1in cancer tissues was significantly higherthan that of pulmonary benign tissues（58.7%vs2.7%）(P＜0.05), and showedsignificant difference with tumor size(52.9%vs73.2%), lymph node metastasis（52.3%vs77.8%）, clinical stages（52.6%vs71.7%）（P＜0.05）; but no significant differencewith age gender, smoking status, differentiated degree and tumor subtype（P＞0.05）. 2.The overexpression rate of CyclinD1in pulmonary adenocarcinoma tissuesshowed significant difference with age（44.8%vs68.4%）, smoking status（52.1%vs76.2%）, tumor size（51.6%vs75.0%）（P＜0.05）; but no significant difference withgender, lymph node metastasis, clinical stages, differentiated degree and tumor subtype（P＞0.05）.3. Loss of P16in non-small cell lung cancer tissues was61.5%as a whole. Therewas no significant difference according to gender, smoking status, clinical stages, tumorsize, lymph node metastasis, tissue typing and differentiated degree（P＞0.05）; butsignificant difference with age（52.9%vs69.9%） and tissue typing（9.1%vs43.8%）（P＜0.05）.4. There was significantly positive correlation between CyclinD1overexpressionand P16loss（P＜0.05）.Conclusion:1. The over-expression rate of CyclinD1in cancer tissues showed significantdifference according to tumor size, lymph node metastasis, clinical stages（P＜0.05）.These results suggested that CyclinD1played an important role in tumorigenesis andprogression by regulating cell cycle.2. The over-expression rate of CyclinD1in pulmonary adenocarcinoma tissuesshowed significant difference with age, smoking status, tumor size（P＜0.05）. whichsuggested that age and smoking status affect the CyclinD1expression inadenocarcinoma.3. Expression of P16in non-small cell lung cancer tissues showed no significantdifference with gender, smoking status, clinical stages, tumor size, lymph nodemetastasis and differentiated degree; but significant difference with age and tissuetyping. On our analysis, p16loss was significantly correlated with the squamous celltype rather than with adenocarcinoma（P＜0.05）.4. There was positive correlation between CyclinD1overexpression of and P16loss. Which suggested that CyclinD1and P16played a cooperative role intumorigenesis and progression by regulating cell cycle. 5. Overexpression of Cyclin D1is highly related to ClinicopathologicalCharacteristics of NSCLCs, it could be a useful biomarker and prognostic factor.6. For these NSCLC with CyclinD1overexpression, targeting cyclinD1could bean effective, cancer-specific therapy.