| Objective: In our study, Vitamin D Binding Protein (VDBP) level in serum and urine ofpatients with type2diabetes was detected in order to analysis the correlation of VDBPand diabetic nephropathy and to provide a new basis for the diagnosis of diabeticnephropathy.Methods: A prospective survey was made in34inpatients (17males and17females)with type2diabetes and11healthy people (5males and6females) from the outpatientswho attended to the Second Affiliated Hospital of DaLian Medical University from Nov2013to Feb2014, which were selected as experimental group and healthy controlsrespectively. According to urinary albumin creatinine ratio (UACR), which wasrecommended by American Diabetes Association (ADA), the patients in experimentalgroup were divided into three groups: normal albuminuria group, microalbuminuriagroup and clinical albuminuria group, UACR is less than30mg/g, between30mg/g to300mg/g, more than300mg/g respectively. Levels of VDBP in every subject’s serumand morning urine were detected by ELISA method. In order to analysis VDBP’srelation with some biochemical indicators as following: fasting plasma glucose (FPG),glycosylated hemoglobin (HbAlc), blood urea (UREA), serum creatinine (Scr),cholesterol (CHOL), triglyceride (TRIG), high-density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C), blood albumin (ALB), morning urinealbumin (UALB) and morning urine creatinine (Ucr), they were also detected. Results:1. Compared to healthy controls, serum VDBP levels in patients with type2diabeteswere significantly higher (P<0.05), but there was no obvious difference between thesethree groups. Serum VDBP had no significant correlation with FPG, HbAlc, UREA, Scr,CHOL, TRIG, HDL-C, LDL-C, ALB, UALB and Ucr.2. Compared to healthy controls, levels of urine VDBP (uVDBP) and urine VDBP/Cr(uVDBP/Cr) in patients with type2diabetes were significantly higher (P<0.05) thanthe healthy controls, and there was an increasing trend in normal, micro-and clinicalalbuminuria group (P<0.05). Levels of uVDBP had no significant correlation with FPG,HbAlc, CHOL, TRIG, HDL-C, LDL-C and ALB, while it was proportional to UALB,UREA, Scr, and inversely proportional to ALB.3. In patients with type2diabetes, uVDBP was negatively correlated with eGFR (r=0.413, p=0.413).Conclusions: Monitoring uVDBP level can reflect the severity of diabetic nephropathy. |
