Research On The Association Of TNFRSF1B Gene Polymorphism And STNFR2with Coronary Heart Disease

AIM:To investigate the relationship between the single nucleotide polymorphism (SNP) TNFRSFIB of gene and soluble tumor necrosis factor receptor, with coronary heart disease (CHD) in Chinese population.METHODS:The polymerase chain reaction (PCR)and ligase detection reaction(LDR) were used to detect TNFRSFIB genotype of SNP rs1061622、rs1061624and rs3397in the208controls and416patients with coronary heart disease (CHD). The levels of plasma sTNFR2was measured by enzyme-linked immunosorbent assay (ELISA) in CHD group and control group,which analysed the association of TNFRSFIB gene polymorphism.To evaluate the association of TNFRSFIB gene polymorphism with CHD susceptibility.RESULTS:The frequency of rs1061622TG+GG or G allele had significant difference between CHD and control (P<0.05).Both the frequency of rs1061624GA+AA or A allele and the frequency of rs3397CT+TT or T allele had not significant difference between CHD and control (p>0.05). These results was independent of age, gender, hypertension, diabetes and hyperlipidemia.The frequency of this polymorphism was consistent with the law of Hardy-Weinberg. The level of plasma sTNFR2in CHD group was significantly higher than in control group.No relationship between TNFRSF1B gene polymorphism and sTNFR2was observed.CONCLUSION1. The rs1061622polymorphism of TNFRSFIB gene might associated with the disease of coronary heart disease (CHD) in Chinese. The rs1061624and rs3397polymorphism of TNFRSFIB gene might not be related to the disease of coronary heart disease (CHD) in Chinese.2. Elevated levels of plasma sTNFR2may be a new risk factor on coronary heart disease.3.The levels of sTNFR2was not significantly associated with TNFRSFIB gene rs106162genotype.