| Objective:To investigate the expression and clinicopathological significance of P-catenin and TIPE2(Tumor necrosis factor-alpha-induced protein-8like-2, TNFAIP8L2) in colorectal cancer.Methods:The expression of P-catenin and TIPE2in49colorectal cancer tissues and27normal tissues were detected by immunohistochemistry. The relationship between their expression and cancer stage, lymph node metastasis and patient survival time were analyzed.Results:1. The positive substance of p-catenin protein is granular in yellowish-brown. Among the27cases of para-carcinoma tissues, the P-catenin protein expression of24cases is localized on the cell membrane, and the cytoplasm of three cases has weak staining, among which the nuclear staining of1specimen is positive. Among49cases of colorectal cancer tissues, the P-catenin protein of14cases is localized on the cell membrane, and the P-catenin protein of35cases has abnormal expression. The abnormal expression (71.43%) of P-catenin in colorectal cancer tissues is higher than that of para-carcinoma tissues (11.11%), and their difference is statistically significant (P<0.05, x2=25.333). Among the7cases of stage Ⅰ colorectal cancer tissues, the P-catenin protein of4cases has abnormal expression,10cases among the17cases of stage ⅡA and ⅡB colorectal cancer tissues, and21cases among the25cases of stage ⅢA and ⅢB colorectal cancer tissues. The abnormal expression of P-catenin protein in stage Ⅰ, Ⅱ and Ⅲ colorectal cancer tissues is57.14%,58.82%and84%respectively. The comparative difference between them is not statistically significant (P>0.05). Among the4cases of high differentiation colorectal cancer tissues, the P-catenin protein of3cases has abnormal expression,27cases among the39cases of middle differentiation colorectal cancer tissues, and5cases among the6cases of low differentiation colorectal cancer tissues. The abnormal expression of P-catenin protein in high differentiation, middle differentiation and low differentiation colorectal cancer is75.00%,69.23%and83.33%respectively. The comparative difference between them is not statistically significant (P>0.05). Among the14cases of colorectal cancer patients with cell membrane expression who have been followed up for5years,11cases are still alive, and23patients among the35colorectal cancer patients with abnormal expression who have been followed up for5years are still alive. The average survival life of the49cases of colorectal cancer patients is50.388months (95%CI:45.538-55.237), among whom the colorectal cancer patients with cell membrane expression survive for52.714months on average (95%CI:44.999-60.429) and the patients with abnormal expression survive for49.457months (95%CI:43.437-55.477). The difference in the survival rate of the two groups by K-M analysis is not statistically significant (P>0.05). Among the25cases of colorectal cancer tissues with lymph node metastasis, the β-catenin protein of21cases has abnormal expression, and14cases among the24cases of colorectal cancer tissues without lymph node metastasis. The abnormal expression (84.00%) of β-catenin in the colorectal cancer tissues with lymph node metastasis is higher than that of the colorectal cancer tissues without lymph node metastasis (58.33%), and their difference is statistically significant (P<0.05, x2=3.953).2. The positive staining of TIPE2protein in colorectal cancer tissues is granular in yellowish-brown, all of which is localized in the cytoplasm. Among the49cases of colorectal cancer,41cases have positive expression while8cases have negative expression. Among27cases of para-carcinoma tissues,12cases have positive expression while15cases have negative expression. The expression (83.67%) of TIPE2protein in colorectal cancer tissues is higher than that (44.44%) of para-carcinoma tissues and their difference is statistically significant (P<0.05,x2=12.684). Among the7cases of stage I colorectal cancer tissues, the TIPE2protein of5cases has positive expression,12cases among the17cases of stage ⅡA and ⅡB colorectal cancer tissues, and24cases among the25cases of stage ⅢA and ⅢB colorectal cancer tissues. The positive expression of TIPE2protein in stage Ⅰ, Ⅱ and Ⅲ colorectal cancer tissues is71.43%,70.59%and96%respectively. The comparative difference between them is not statistically significant (P>0.05). Among the4cases of high differentiation colorectal cancer tissues, the TIPE2protein of3cases has positive expression,32cases among the39cases of middle differentiation colorectal cancer tissues, and6cases among the6cases of low differentiation colorectal cancer tissues. The positive expression of TIPE2protein in high differentiation, middle differentiation and low differentiation colorectal cancer is75.00%,82.05%and100%respectively. The comparative difference between them is not statistically significant (P>0.05). Among the8cases of colorectal cancer patients with negative expression of TIPE2protein who have been followed up for5years,6cases are still alive, and28patients among the41colorectal cancer patients with positive expression who have been followed up for5years are still alive. The average survival life of the49cases of colorectal cancer patients is50.388months (95%CI:45.538-55.237), among whom the colorectal cancer patients with positive expression of TIPE2protein survive for49.098months on average (95%CI:43.448-54.747) and the patients with negative expression survive for57.000months (95%CI:52.659-61.341). The difference in the survival rate of the two groups by K-M analysis is not statistically significant (P>0.05). Among the25cases of colorectal cancer tissues with lymph node metastasis, the TIPE2protein of24cases has positive expression, and17cases among the24cases of colorectal cancer tissues without lymph node metastasis. The positive expression (96.00%) of TIPE2protein in the colorectal cancer tissues with lymph node metastasis is higher than that of the colorectal cancer tissues without lymph node metastasis (70.83%), and their difference is statistically significant (P<0.05, x2=3.984).Conclusion:The abnormal expression of β-catenin and the positive expression of TIPE2may play an important role in the occurrence and development o f colorectal cancer. |
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