Effects Of Conantokin-Ganalogues Inhibiting Morphine Induced CPP And The NNOS Expression In Mice

11ObjectiveThis study examined the effects of Glu-Con-G、Glu-Con-G[1-11] and Glu-Con-G[S16Y]on morphine dependence, providing the direction to design the structure of Conantokin-G analogues. To explore the role of nNOS in morphine psychological dependence, we detected the changes of the levels of nNOS in hippocampal region of morphine-addicted mice by used the western blotting.2Experimental approachesMale Kunming mice (18-20g) were used in this experiment. The experiments included two parts:behavioral experiment (CPP) and the biochemistry experiment (Western blotting).3ResultsGlu-Con-G (120、240、480、960pmol), Glu-Con-G[1-11](120.240,480pmol) and Glu-Con-G[S16Y](480,960pmol) inhibited the expression and reinstatement phases of morphine-induced CPP (conditioned place preference), but had no significant behavioral disorder under the experimental conditions.The results of western blotting showed that, certain doses of Glu-Con-G (120、240、480、960pmol)、Glu-Con-G[1-11](120、240、480pmol) and Glu-Con- G[S16Y](240、480、60pmol) can attenuate the levels of nNOS in Hp brain ar eas of morphine addictive mice.4ConclusionsThese results indicate that three Conantokin-Ganalogues can inhibit morphine dependence. They are expected to become potential anti-addictive drugs.The levels of nNOS were increased in Hp brain areas of morphine addictive mice. The certain doses of Conantokin-G analogues can attenuate the change.