Effects Of Intravenous Injection Glu-Con-G And Glu-Con-G[1-13] On Morphine Induced Physical Dependence And Rewarding In Mice

1ObjectiveIn the present study, we study the effects of intravenous injection Glu-Con-G and Glu-Con-G[1-13] on sleeping and morphine induced physical dependence through establishing psychological and physical dependence model, and we further focus on the anti-morphine psychic dependence effects of intravenous injection Glu-Con-G and Glu-Con-G[1-13]by CPP.2Experimental approachMale Kunming mice (18-20g) were used in this experiment. And the experiment was made up of three parts:hypnotic experiment, physical dependence experiment and behavioral experiment (CPP).3ResultsThe results of hypnotic experiment shows that, Glu-Con-G and Glu-Con-G[1-13](200,400,800μg/kg)by intravenous injection had no effect on the sleep in mice(P>0.05); but could significantly reduce the latent period after pentobarbital sodium injection(P<0.05), and Glu-Con-G and Glu-Con-G[1-13](400,800μg/kg)prolong the time of sleep induced by pentobarbital sodium injection(P<0.05).The results of physical dependence experiment shows that, Glu-Con-G and Glu-Con-G[1-13](200,400,800μg/kg)by intravenous injection could inhibit the physical dependence (number jumps&body weight loss) induced by morphine (P<0.05). The results of behavioral experiment(CPP)shows that, Glu-Con-G and Glu-Con-G[1-13](200,400,800μg/kg)by intravenous injection attenuated the expression phases of morphine-induced CPP(P<0.05), but had no effect on reinstatement phases and had no invisible behavioral disorder under our experiment conditions(P>0.05).4ConclusionsIntravenous injection Glu-Con-G[1-13] have better effect than Glu-Con-G on prolonging the sleep and inhibiting physical dependence induced by morphine, both Glu-Con-G and Glu-Con-G[1-13] could attenuated the expression phases of morphine-induced CPP.