| Objective Using Langendorff isolated heart perfusion model to explore the TibetanSanweitanxiang Powder on the hypoxic isolated rat heart ischemia-reperfusion injury,in order to explore its mechanism of action.Methods Simulated altitude of5000m hypoxia20d, Wistar rats were given highdose Sanweitanxiang powder (High Dose), low-dose group (Low Dose) processing18d. Isolated Langendorff heart perfusion system in vitro cardiacischemia-reperfusion of each group performed were recorded stable perfusion andreperfusion end system of perfusion pressure, left ventricular systolic pressure (LVSP),left ventricular pressure maximum/minimum rate of change (±Dp/Dtmax) and heartrate (HR) changes in the situation, while collecting various cardiac perfusion fluidlactate dehydrogenase (LDH), creatine kinase (CK) activity, and myocardialsuperoxide dismutase (SOD), malondialdehyde (MDA) and trace type glutathione(GSH) content changes, and observe heart tissue morphology, investigateSanweitanxiang powder hypoxic isolated rat heart ischemia-reperfusion injury andmechanism.Results Observed each group’s cardiac dynamics indicators can found that, thereperfusion period of relatively stable perfusion pressure was significant (P <0.05),the absolute value of LVSP and±dp/dtmaxwere decreased significantly (P <0.05),the heart rate did not have significant change. The normoxic low-dose group’sabsolute value of LVSP and±dp/dtmaxwere significantly higher than normoxiccontrols (P <0.05), in the reperfusion period. The hypoxia small doses and the controlgroup’s absolute value of LVSP and±dp/dtmaxwere significantly higher than that ofhypoxia (P <0.05); in reperfusion period. There was no significant difference betweenthe stable and reperfusion of indicators, hypoxic and normoxic control group, hypoxicand normoxic high-dose groups, while hypoxia small dose’s absolute value of LVSPand±dp/dtmaxwere significantly higher than normoxic low-dose group. Cardiac perfusion biochemical indicators:1the content of CK, LDH and MDA weresignificantly higher than the stabilization period (P <0.05), in the reperfusion periodof each group. In normoxic and hypoxic group, the drug dosage groups indicatorsincreased rate slowed compared with the control group, suggesting that the drugsdone. The change of indicators in low-dose group more obvious than the larger dosegroup (P <0.05), showes the effect of low-dose group in the hypoxic more highlightthan normoxia small dose group (P <0.05).2The content of T-SOD and GSH weedecreased in the reperfusion period than stable period, and the difference wassignificant (P<0.05). In normoxic and hypoxic group, the drug dosage groupsindicators increased rate were slower than the control group, suggesting that thedrugs done. The small dose of drug targets to improve the situation is more obvious(P <0.05), and the effect of low-dose group more prominent in hypoxic than normoxiclow-dose group (P <0.05).3Histological examination shows that: compared withthe hypoxia control group, the hypoxia drug group’s myocardial stripes are clear、muscle don’t have soluble and myosin、 partially visible compensatory stromalvascular recanalization, suggesting that the protective effect of the drug.Conclusion1Sanweitanxiang powder can reduce the heart injury fromischemia-reperfusion and has the cardiac effect.2Sanweitanxiang powder can reducemyocardial CK、LDH and MDA, increase the content of T-SOD and GSH, improvecardiac morphological changes,that may be the protective mechanism for the heartmuscle. |
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