| Objective The purpose of this study was to investigate the protective effects of SanWeiTanXiang powder on anesthetized rat hearts against myocardial ischemia and reperfusion injury and its possible mechanism. Methods A model of regional myocardial ischemia-reperfusion(IR) injury was established by 30min ligation of the left anterior descending coronary artery followed by 40min reperfusion in hearts of anesthetized rat. The experimental rats were randomly divided into the sham operation(SO), IR model group,positive control group and two dose SanWeiTanXiang powder groups(1.0g.kg-1& 1.5g.kg-1).The ECG ST segment,and heart rate(HR)were observed in the vriouse expreiment groups.The hemodynamics including LVSP,±DP/DTmax,LVEDP and MBP were measured by American Biopac System(Biopac Mp150)during the period of ischemia and reperfusion.Serum concentrations of LDH and CK were measured.The levels of SOD,GSH-PX,NOS,iNOS,MDA,and NO in the myocardium tissues were measured.Myocardial ultrastructure were examined by light and electronic microscope for evaluating the cardio protective effect.Results There was significant less displacement of ST in the pretreated with SanWei TanXiang group which compared to the IR group,and the values of MBP,LVSP,±DP/DTmaxand LVEDP were markedly higher in the pretreated group than that of the IR model group. The level of LDH,CK were decreased,the activities of SOD and GSH-PX in myocardium in the group of pretreated with SanWeiTanXiang powder were significantly higher than those in IR model group.The concentration of MDA,NOS,iNOS,NO in myocardium were less than those in the IR model group.The result of myocardial ultrastructure indicated that the partial mitochondria crista and myofibrils sprase,z band unaltered compared with model group that the most of mitochondria swelling and interstitial edema,myofibrils and z band altered.Conclusion SanWeiTanXiang powder play a significant protective role against myocardial ischemia and reperfusion injury of rats.It may be protect the structure and function of mitochondria,anti-oxidation and reduce the NO over release. |
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