The Preparation Of FCM Single Cell Suspensions What’s From Paraffin-embedded’s Colorectal Tumor In Lymphoey

Purpose: The single cell suspensions of colorectal tumor infiltrating lymphocytes weresuccessfully prepared by the treatment of paraffin-embedding tissue.For further analysis incolorectal cancer lymphocyte immune state and colorectal cancer patients withpathological characteristics, the connection between the clinical stage and prognosis,thecells was detected by flow cytometry.Methods:60cases of colorectal cancer paraffin-embedding samples were collected fromAffiliated Hospital of Zunyi Medical College general surgery in June1990to June1990.After a slice, dewaxing, hydration, digestion, filtration, lymphocytes were detectedby flow cytometry.The results was analyzed for the study of colorectal cancer lymphcellular immune state and colorectal cancer pathological characteristics, and therelationship between the clinical stage and prognosis.Results: The cells through the preparation of single suspended cells on microscopy wasfound to form a complete, good dispersion, less cell stack and debris, cleanbackground.More than10cells were counted in each grid through blood plate.The resultsfrom flow cytometry are satisfied.Conclusion: The single cell suspensions of colorectal tumor infiltrating lymphocytes weresuccessfully prepared by the treatment of paraffin-embedding tissue.The data of the flowcytometry was corrected detected. The analysis of colorectal cancer lymphocyte immunestate and pathological characteristics, the relationship between the clinical stage andprognosis is of practical significance.