Changes Of CD4,CD8 And CD28 T Cells In Colorectal Cancer And Adenoma And Its Clinical Significance

Objective: To study the expression of CD4,CD8 and CD28 T cells in colorectal cancer(CRC),colorectal adenoma,and to study the relationship and significance between T lymphocyte subsets(TLS)and colorectal cancer.It was hoped that the corresponding TLS such as CD4,CD8 and CD28 could be used as a reference basis for early detection and intervention of colorectal tumors.Methods:(1)The tissues of 21,20 and 20 cases from normal large intestine(normal group),colorectal adenoma(adenoma group),CRC(cancer group)were strictly screened and selected as experimental samples.Immunohistochemistry(IHC)staining was used to detect the expression of CD28 in TLS in these three colorectal specimens to explore the correlation between clinical data of CD28 and CRC.(2)TLS such as CD4,CD8 and CD28 were detected by flow cytometry in peripheral blood of 20 healthy individuals(normal group),10 colorectal adenomas(adenoma group),30 CRC(cancer group)and the correlation between clinical data of CRC and TLS was also analyzed.Results:(1)The positive rates of CD28+T cells in normal group,adenoma group and cancer group were 57.14%,30.00% and 20.00% respectively.There was a statistically significant difference in the positivity of CD28+T cells between different tissues(p < 0.05).CD28+T cells were not associated with gender,age,lymph node metastasis,and were associated with tumor location,stage,and distant metastasis.(2)The proportions of CD4+,CD8+,CD4+/CD8+,CD28+,CD8+CD28+,and CD8+CD28-T in the peripheral blood,the adenoma group and the cancer group were generally different(P<0.05).Conclusions:(1)The expression of CD28+T cells decreased gradually during the evolution of "normal-adenoma-carcinoma".CD28+T cells are related to the location,stage and distant metastasis of the tumor.It suggests that CD28+T cells can provide a basis for the study of the condition and prognosis of CRC patients.(2)The proportions of CD4+,CD8+,CD4+/CD8+,CD28+,CD8+CD28+T,and CD8+CD28-T in the normal group,adenoma group and cancer group in the CRC microenvironment are generally different from those in the normal population.This suggests that changes in the proportion of T cell subsets are associated with CRC progression.