The Used Effective Time Of The Disinfection Of Machines’ Breathing-circuit-systems And Analysis Of The Related Factors Associated With The Contamination Of Breathing-circuit-systems

Objectives1. To understand anesthesia machines’breathing-circuit-systems, contamination2. After disinfection of anesthesia machines’ breathing-circuit-systems, to explore theeffective time, provide a reference of disinfection of clinical anesthesia machines3. To study the related factors of the contamination of internal circuits of anesthesiamachine. The results could provide a reference for hospitals to formulate correspondingmanagement measures.Methods：1. Ohmeda anesthesia machine from our hospital were enrolled in the study. Beforedisinfecting machines samples were taken from7defined locations inside the anesthesiamachine’s internal breathing-circuit-systems: the inspiratory port,、the expiratory port、theinspiration unidirectional valve, the expiration unidirectional valve, bellows and the tworubber gaskets located towards the CO2absorber. To understand anesthesia machines’ breathing-circuit-systems,contamination2. After the machines were disinfected, the treated group were used for7days andsamples were taken from7defined locations each day. The control group were not usedfor15days and samples were taken from7defined locations each day. Use survivalanalysis to explore the effective time.3.904patients were studied. Recorded the patients, gender, age, diagnosis, endoscopicsurgery, cardiopulmonary bypass, surgical departments, tracheal intubation generalanesthesia, operation time, operative position, pulmonary function, operation roomnumber, anesthesia machine, et al。The data was inputted to SPSS18.0, analysis riskfactors of anesthesia machines’internal breathing-circuit-systems，the contaminationResult1. The gram-negative bacteria was accounted for52.96%, which there wereacinetobacter baumannii(68)and pseudomonas aeruginosa(37). Gram-positive bacteria wasaccounted for32.41%, which there were Corynebacterium diphtheria (29), Streptococcuspneumonia(26), Streptococcus viridans (20), Staphylococcus aureus(9),coagulase-negative staphylococci(5), Corynebacterium diphtheria(3), Bacillus subtilis(1)2. We found that viable bacteria were in breathing-circuit-systems,245sampled fromdefined locations. the inspiratory unidirectional valve had massive contamination. Thecontaminated locations were the import of the CO2absorber (22.86%,), the inspiratoryport(22.45%), bellows (13.47%), the export of the CO2absorber (8.57%), the expirationunidirectional valve (2.04%),the expiratory (1.22%)3. The effective time after disinfecting internal circuits of anesthesia machine was7d4. Univariate analysis showed that age, tracheal intubation general anesthesia,endoscopic surgery, surgical departments, operation time, and operative position weresignificantly associated with the the contamination (P <0.05).5. Multivariate analysis showed that age, endoscopic surgery, operation time, proneposition, lateral position were independent risk factors for the contamination (P <0.05).Conclusion1. anesthesia machines’ breathing-circuit-systems contamination became risk factors for nosocomial infections, the medical staff should pay attention to this problem。2. The effective time after disinfecting internal circuits of anesthesia machine was7d3. Age, endoscopic surgery, operation time, prone position, lateral position wereindependent risk factors for the contamination. Proactive prevention and control measuresagainst risk factors should be taken to reduce the risk of nosocomial infection...