The Significance Of The Combined Measurement Of Serum MIC-1、CEA、CA724、CA50in Gastric Cancer

Objective: To study the expression level of macrophage inhibitory cytokine-1(MIC-1)in serum of gastric cancer patients, and its correlation with clinicopathologicalfeatures of gastric cancer, and the significance of combined detection of MIC-1、CEA、CA72、CA50in the diagnosis of gastric carcinoma.Methods: The blood samples of patients who were confirmed pathologicallyhospitalized in Affiliate Hospital of Qinghai University. A total of199cases, dividedinto three groups, one group of gastric cancer141cases, gastric benign disease groupof29cases,29cases of normal control group. ELISA method was used to detect theexpression level of MIC-1in serum, CA50was measured by immunoradiometricassay and CEA、CA724were detected by electrochemiluminescence immunoassay. Tocompared the expression levels of MIC-1and CA50、CEA、CA724in the serum. Toanalyze expression level of MIC-1in gastric cancer group, gastric benign diseasegroup and normal control group through the Mann-Whitney U test. The relationshipbetween MIC-1and clinicopathological features in gastric cancer group was analyzed.According to the tumor marker values to statistical analysis, and draw the ROC curve.According to the area under the ROC curve shows that the sensitivity of tumormarkers. Detected the sensitivity of MIC-1, and analyzed the sensitivity of combineddetection of MIC-1and CEA, CA724, CA50.Results:1. MIC-1level in gastric cancer was significantly higher than that in benigngastric disease group and normal control group (P<0.001), no significant difference ofgastric benign disease group and normal control group (P>0.05).2. Significant difference in expression of MIC-1was found between patientswith TNM stages (P<0.001), and no significant difference between patients with age,gender, tumor site and differentiation degree (P>0.05). 3. The area under the ROC curve of MIC-1was closer to1, the area under thecurve is better than that of CEA, CA724, CA50and the sensitivity of MIC-1may besuperior to CEA, CA724, CA50. In serum of gastric cancer patients MIC-1、CEA、CA724and CA50Ⅰ-Ⅳ area under the ROC curve respectively were：MIC-1：1，1，1，1；CEA：0.4236，0.8943，0.9224，0.9575；CA724：0.5665，0.9098，0.9126，0.9594；CA50：0.6552，0.8517，0.8143，0.8973. Area under the ROC curveof MIC-1, CEA, CA72-4and CA50increased with clinical stage increasing.4. The sensitivity of MIC-1, CEA, CA724and CA50in gastric cancer patientswere88.7%，63.1%，71.6%，36.2%respectively. The sensitivity of combineddetection were90.7%、92.9%、90.8%respectively. Sensitivity of four kinds of tumormarkers for combination detection was96.4%. Consequently, four kinds of tumormarkers joint detection can improve gastric cancer diagnosis.Conclusions: MIC-1is a new effective gastric cancer tumor maker. It wassignificantly increased in serum, and decreased after treatment. The expression levelof MIC-1in serum increased with clinical stage increasing. MIC-1can reflect thedynamic changes in patients with tumor. The combination detection of tumor markersin gastric cancer have important clinical value in improving diagnosis, reducing theratio of failure diagnosis，monitoring curative effect. It can provide reference forclinical practical work.