Preparation Of Passiflora Edulis Sims Pectin And Its Regulation On Ulcerative Colitics Bioactivity

The subject was performed on the investigation of purple fruit Passiflora edulis peel.Mainly aiming at fully exploiting and utilizing purple fruit Passiflora edulis processing wastesin order to improve the product added value and provide preliminary research informationabout activity and health care function of pectin polysaccharides through conducting aresearch on extraction, decoloration, skimming protein and fat and polysaccharidesenrichment technology. Meanwhile, the subject performed on study of pectin polysaccharidebasic composition, properties of pectin polysaccharide structure and mucosal healing ofulcerative colitis rats.Weighing method, ultraviolet spectrophotometer determination, titration determinationwas used to determinate Rate of dry weight, galacturonic acid content and methyl degrees.Microwave, Ultrasonic and different kinds of mixed acids on purple fruit passion fruit pectinpolysaccharide extraction was compared, the best mixed-acid was chosen, while responsesurface and Box-Behnken experimental design method was used to optimize the extractionprocess; By comparison, UV spectrophotometric determination, coomassie brilliant bluemethod, Sulphuric acid-carbazole method was used, after Static and dynamic adsorptionexperiments, polyamide resin was utilized in decolorization and its process was optimized;eluent concentration for DEAE-cellulose52enriching polysaccharides process was alsodetermined by coomassie brilliant blue and Sulphuric acid-carbazole method; dry decrementmethod, coomassie brilliant blue method, soxhlet extraction method and acid carbazolemethod were used to determine pectin polysaccharide composition, high gel filtrationchromatography was used to determine the molecular weight size, GC-MC was used formonosaccharide composition determination, titration analysis and infrared spectrometry wereused to analyse structure of pectin polysaccharide; ulcerative colitis rats model was made byTNBS-ethanol, and pectin polysaccharide effect on ulcerative colitis mucosa of rats wasstudied by signs of state, evaluation index, HE staining and ELISA of inflammation factors.Extraction results showed that hydrochloric acid obtain highest pectin yield, citric acidobtain highest esterification degree, while citric acid and hydrochloric acid mixed solutioncan obtain both high ester and high extraction rate. Box-Behnken response surface method showed that under the condition of30%citric acid and hydrochloric acid mixing in ratio of2:1, material liquid1:40(w:v), pH1.5, temperature65℃, the extraction time of1h, the highestpectin polysaccharide yield was10.98%, which was extremely closed to theoretical value of10.95%.And its methyl degree was63.77%.In purification process, static adsorption of resin in decolorization process showed thatpolyamide resin occupied the best advantage in decolorization with high rate ofpolysaccharide retention and protein removal. Result of optimization in dynamic polyamideresin decolorization process by Box-Behnken response surface showed that on condition that:sample volume was3.5BV, flowing rate was3BV/h, ph3.5, the decolorization andpolysaccharide retention efficiency were the best, which was53.41%and87.20%, closing topredicted value54.91%and88.30%.1BV sample volumn and0.3mol/L Nacl as the eluentwere the best in DEAE-cellulose52resin enrichment of pectin polysaccharides, dry weightrecovery rate was84.00%and galacturonic acid content was80.32%.Passiflora edulis pectin polysaccharide properties identification showed that theproperties met GB25533-2010criterion. The material composition comparison results turnedout that with purification process caring through, yield of pectin polysaccharide decreased,but its purity was improved including color and impurities reducing. The pectinpolysaccharide coming from mixed acids extraction, decoloration and DEAE-cellulose52resin purification technology containing fat (0.17%), protein (0.26%), acid insoluble ashcontent (0.47%), moisture (9.08%), galacturonic acid (80.32%).And it was composed withribose(4.41%), rhamnose (0.17%), arabinose (3.53%), xylose (0.72%), mannose(1.34%),glucose(4.65%), galactose(3.84%). Structure research showed that the methyl degree was71.60%, molecular weight was307.86KD, and dispersion coefficient degree was3.76. It canbe conclude that the extracted pectin polysaccharide containing low fat, protein, acidinsoluble ash content, moisture and having high purity, molecular weight and ester pectin, wasa good source of pectin polysaccharides and had a valuable exploitation potential.Healing mucosa activity of Passiflora edulis pectin polysaccharide in ulcerative coliticsrats research showed that oral pectin polysaccharide80mg/kg-100mg/kg per day can upgradeIL-10and downgrade TNF-α andIL-1β, as well as decrease mucosa impair, which means, to acertain extent, passiflora edulis pectin polysaccharide can reduce the occurrence and development of ulcerative colitis.In a conclusion, Hydrochloric acid-30%citric acid method can obtain higher yield ofhigh ester pectin, and the process is simple; Polyamide resin can be used for the purple fruitpassion fruit pectin polysaccharides decolorization, which can effective decolor and eliminateprotein with high retention rate of galacturonic acid, and its process is simple as well; DEAE-cellulose52column can effectively purify and enrich pectin polysaccharides; mixed acidmethod can obtain low fat, protein, acid insoluble ash content, moisture, but high purity andmolecular weight of high ester pectin, and Passiflora edulis Sims is a good source of pectinpolysaccharides; pectin polysaccharides of passiflora edulis Sims peel can improve colonicmucosal inflammation of ulcerative colitis rats by regulating inflammation factors.