Based On HGF/c-Met Signal Pathway Study On The Mechanism Of The QHF Formula In Combination With Cisplatin Against The Mouse Model For Primary Hepatic Carcinoma

Purpose: This study evaluates the effect of the herbal compound recipe “QHF” andDDP on hepatocellular carcinoma (HCC). To explore the mechanism of compound recipeon HCC.Method:60mice were randomly divided into the normal group (12mice), the modelgroup(12mice), the QHF group(12mice), the DDP group(12mice) and the QHF plus DDPgroup(12mice). An orthotopic xenograft tumor model of hepatocellular carcinoma wascreated by injection of H22cells directly into the liver parenchyma of BALB/c mice. Thenormal group was created by injection of NS. The inhibitory effects of these drugs ontumor growth were evaluated by calculating the reduced rate of tumor growth. The survivalcondition of mice was examined by observing the general condition of mice and the spleenindex, thymus index. The morphological changes of tumor cells were observed under anoptical microscope. The AST, ALT and AFP in blood serum was evaluated by kit. TheHGF、c-Met, p-c-Met, p38, p-p38, ERK, p-ERK, VEGF in tumor and liver tissue wasevaluated by Western Blot.Result:(1) The tumor weight was significantly lower in the QHF group, DDP group,and QHF plus DDP group than in the Model group (0.24g±0.07g,0.18g±0.03g and0.14g±0.01g VS0.38g±0.05g, respectively; P <0.05or P <0.01).(2) The reducedrates of tumor growth in the QHF group, DDP group and QHF plus DDP group were38.7%,52.6%and63.5%, respectively. The AST, ALT, AFP in blood serum wassignificantly lower in the QHF group, DDP group, and QHF plus DDP group than in themodel control group (P <0.05or P <0.01).(3) The HGF, c-Met, p-c-Met, p-p38, p-ERK,VEGF in the tumor tissue was significantly lower in the QHF group, DDP group, and QHFplus DDP group than in the model control group P <0.05or P <0.01).(4) Compared withthe DDP group, a lower incidence of toxic reactions and a better quality of survival wereobserved in the QHF plus DDP group than in the DDP group.Conclusion: Both of QHF and DDP have anti-angiogenic effects in H22hepatocellular carcinoma in mice. QHF in combination with small-dose DDP hassynergistic anti-angiogenic effects and can improve the quality of survival and reduce theincidence of toxic reactions in mice bearing H22hepatocellular carcinoma. QHF caninhibit the tumor growth. It could inhibit the c-Met, P38, ERK, VEGF signal pathway.