Study For Relationship Between The Polymorphism Of Endothelial Nitric Oxide Synthesis（eNOS）gene G894t、angiogenesis Ⅱtype1Receptor (AT1R) Gene A1166C And Essential Hypertension (EH) In Hui And Han Nationality Of Ningxia Province, China

Objective To investigate the association between the polymorphism ofendothelial nitric oxide synthesis gene G894T and essential hypertension（EH）inHui and Han nationality in Ningxia Province, China.Methods The genomic DNA was extracted from the peripheral blood of588peoples in Ningxia Region. Polymerase chain reaction (PCR) and restrictionfragment length polymorphism (RFLP) methods were used to detect the G894Tpolymorphism of eNOS gene in Hui nationality including142patients with EHand112normal controls. The PCR-RFLP methods were used to in Han nationalityincluding158patients with EH and176normal controls.The frequencies ofgenotype distribution in Hui and Han nationality of essential hypertension inNingxia were studied.Results (1) Hui nationality:The distribution genotype frequencies of GG,GT and TT were83.10%,16.20%and8.80%in hypertension groups, and those innormal groups were85.71%,14.29%and0, respectively, which showed nosignificant difference(P>0.05); The allelic frequencies of G and T were91.20%,8.80%respectively in hypertension group and92.86%,7.14%in normalgroup. The allele frequencies of G/T were no significant differences to abovedata in two groups (P>0.05).(2) Han nationality: The distribution genotypefrequencies of GG, GT and TT were86.71%,12.66%and0.63%in hypertensiongroups, and those in normal groups were87.50%,11.36%and1.14%, respectively, which showed no significant difference (P>0.05); the allelic frequencies of G andT were93.04%,6.96%respectively in hypertension group and93.18%,6.82%innormal group. The allele frequencies of G/T were no significant differences toabove data in two groups (P>0.05).Conclusions It may not be association between the G894T polymorphismof eNOS gene and essential hypertension in Hui and Han nationality of Ningxia.There also does not exist racial difference between Hui and Han people with eNOSG894T gene polymorphism. Objective To investigate the association between the polymorphism ofangiogenesis Ⅱ type1receptor (AT1R) gene A1166C and essential hypertension（EH）in Hui and Han nationality in Ningxia Province, China.Methods The genomic DNA was extracted from the peripheral blood of594peoples in Ningxia Region. Polymerase chain reaction (PCR) and restrictionfragment length polymorphism (RFLP) methods were used to detect the A1166Cpolymorphism of AT1R gene in Hui nationality including146patients with EHand112normal controls. The PCR-RFLP methods were used to in Han nationalityincluding160patients with EH and176normal controls.The frequencies ofgenotype distribution in Hui and Han nationality of essential hypertension inNingxia were studied,and the relationship between the polymorphism ofangiontensinⅡ type1receptor（AT1R） gene A1166C and dyslipidemia levels inessential hypertensive Hui nationality of Ningxia Province, China.Results (1) Hui nationality: The distribution genotype frequencies of AA,AC and CC were88.36%,11.64%and0in EH group, and those in normal groupwere93.75%,6.25%and0, respectively, which showed no significant difference(P>0.05); The allelic frequencies of A and C were94.18%and5.82%in EH group,and96.87%and3.13%in normal group, respectively (P>0.05). Meanwhile, Therewas no significant difference in the genotype frequencies of AA, AC, CC and theA/C allele frequencies between EH group and normal group (P>0.05). The TG levels are significantly higher in hypertension group than those in normal group[（2.11±1.16） mmol/L vs（1.53±1.01） mmol/L (P <0.001)]; In hypertensiongroup,TG、 TC、 HDL-C and LDL-C are no related to A1166C polymorphism ofAT1R gene,and dyslipidemia levels of AC carriers are no significantly higherthan AA carriers (P>0.05).(2) Han nationality: The distribution genotypefrequencies of AA, AC and CC were93.13%,6.25%and0.62%in hypertensiongroups, and those in normal groups were87.50%,11.36%and1.14%, respectively,which showed no significant difference(P>0.05); The allelic frequencies of Aand C were96.25%,3.75%respectively in hypertension group and93.18%,6.82%in normal group. The allele frequencies of A/C were no significant differences toabove data in two groups (P>0.05).Conclusions There was no significant association between the A1166Cpolymorphism of AT1R gene and EH in Hui and Han nationality of Ningxia.,andno significant association found between the A1166C polymorphism of AT1Rgene and dyslipidemia levels in hypertensive Hui nationality of Ningxia.