| 【Background】 Gastric cancer is one of the most common malignant tumorworldwide, China is a high incidence area of gastric cancer. Mena is a newlydiscovered and genes related to the development of gastric cancer, but Mena specificgene expression patterns in gastric cancer as well as the relationship betweenclinicopathological features is unknown.【Objective】 SNPs Mena expression and biological behavior of gastric cancercorrelation.【Methods】 In January2001December-2009First Affiliated Hospital of FujianMedical University, resected specimens were archived wax blocks, all selectedpatients had complete follow-up data. Select an archive of252cases deaths paraffin,producing tissue microarray by immunohistochemical staining Mena proteinexpression. Selected cases of317cases of normal gastric mucosa paraffin tissue cutends and188healthy subjects whole blood samples, by extracting DNA, PCR-LDRmethod and Mena gene sequencing to detect single nucleotide polymorphisms(SNPs).【Results】1. Mena expressed in normal gastric glandular epithelium, the positive orientationinherent in the gastric glands, the cytoplasm was filled with brown, strong positiveexpression.2. The expression of Mena in gastric carcinoma of mucosa, tumor center, invasivefront in and lymphnode metastasis and the differentiation and histological type(P<0.05). Gastric mucosa, expression of well-differentiated by Mena poorlydifferentiated was significantly higher than those (59.66%VS47.37%), intestinal typegastric cancer Mena expression was significantly higher than that of the high diffuse gastric cancer (59.15VS21.88%). Mena with gender, age, tumor location, depth ofinvasion and lymph node metastasis was no correlation (P>0.05).3. Survival analysis showed that the mucous layer and metastatic lymph nodecarcinoma of high Mena expression had worse prognosis than that of the lower Menaexpression (P <0.05). Mucosa carcinoma: chi-square value=10.5753, degrees offreedom v=3, the probability P=0.0143; lymph node metastasis cancer: chi-squarevalue=10.7081, degrees of freedom v=3, the probability P=0.0134.4, Mena3gene loci rs113170551, rs113271908, rs75986582were homozygous, nodifference in the case group and control group.5, Mena loci rs75136619was A/G heterozygous expression, the heterozygousfrequency in case group and control group no significant difference.6, Mena locus rs3795443A allele frequency in case group was lower than the controlgroup (85.4%vs91.1%), G allele frequency in case group was higher than the controlgroup (14.6%vs9.0%),(P <0.05). AA, A/G, GG genotype frequency in cases andcontrol group (73.3%,24.1%,2.5%vs81.9%,18.1%,0.0%)(P <0.05). Case groupalleles A/G, GG genotype frequency were higher than the control group.7, Mena locus rs3795443G allele frequency distribution in the Group aged＜60-year-old patients was higher than group aged≥60-year-old patients with gastriccancer (18.6%vs11.8%),(P <0.05).8, Mena locus rs3795443genotype frequencies: AA genotype frequency in groupaged＜60-year-old patients was lower than group aged≥60years-old patients withgastric cancer(67.4%vs77.4%), A/G, GG genotype frequency increased (27.9%,4.7%vs21.5%,1.1%),(P <0.05).9, Mena locus rs3795443in the AA, A/G, GG genotype between gastric cancer wasno significant difference in survival time.【Conclusion】1. Mena expression and maintain the structure of gastric cancer maybe participate inthe regulation of tumor differentiation. 2.Prognosis of carcinoma with high expression of Mena in mucosa and lymph nodemetastatic carcinoma may be poor.3. rs3795443G allele frequency of the rare sites of healthy people in Fujian province,was0.090.4. Sites with Mena SNP rs3795443G allele increased risk of gastric cancerindividuals.4. rs3795443G allele frequency distribution was higher in group aged＜60-year-oldpatients of gastric cancer than in group aged≥60years patients.5. Mena SNP site rs3795443genotype not associated with gastric cancer survival. |
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