| According to the existing foundation and resources in this test, in order to five different genetic basis of hybridization goose as research target. The growth and slaughter performance of geese with different genetic basis were detected and compared based on the selection of 5 hybrid groups through feeding in production rest; I also analysis the expression of GHR and MSTN gene in muscle of geese at different growth stages by real-time PCR; The radioimmunassay was performed to detect the content of GH in serum in early development stage. The study also analyzed the correlation among GH, meat production trait and expression characteristic of gene, which will finally provide experimental basis and reference for identifying the genetic markers related to meat production trait and selecting the special line of high quality goose. The results are as follows: 1. Analysis the difference of growth and slaughter performance in different genetic basis geeseUnder the same management condition with trophic level, the growth and development of the geese in 5 experimental groups were basically the same. The daily gain from the early breeding, increased first and then decreased. The growth peak was near the 5th week. At the age of 10 weeks, the marketing weight of group â… was 3334.67 g, group â…¡ was 4047.67 g, group â…¢ was 3798.27 g, group â…£ was 3571.47 g, and group â…¤ was 3814.13 g. The mean weight of group â…¡ was significantly higher than that of other experimental groups(P<0.05). The slaughter rates of five groups of hybrid geese with different genetic basis were all above 87%, the eviscerated rates above 70%. Among these groups, the laughter performance of group â…¡ was most outstanding. At the age of 13 weeks, the down performance of group â…¤ was 128.89 g, was best due to the down yield of group(128.89g), and the down rate(40.09%). 2. The expression analysis of GHR mRNA and MSTN mRNAIn the early growth and development process, the expression of GHR and MSTN mRNA in 5 groups increased first and then decreased, with the same expression law. At the same stage of development, the GHR mRNA and MSTN mRNA in different muscle tissues had different expression levels. The relative expression of GHR mRNA in breast muscle was significantly higher than crureus. The relative expression quantity of GHR mRNA in breast muscle of group â…¡ was significantly higher than that in breast of other experimental groups(P<0.05) at the age of 4 weeks and 10 weeks. The relative expression quantity of MSTN mRNA in crureus and breast muscle at newborn and 4 weeks between crureus and breast muscle had little difference. The relative expression of MSTN mRNA in breast of group â… was significantly higher than that in breast of other experimental groups(P<0.05). At the age of 10 weeks, the relative expression of MSTN mRNA in breast muscle was significantly higher than crureus, and the relative expression of MSTN gene of group â… was significantly higher than that of other four experimental groups(P<0.05). 3. The correlation analysis among the expression of GH, meat production trait and the expression of gene:The expression regularity of GH, GHR, MSTN was identical with the early development regularity of 5 experimental groups. The expression of GH in serum showed significant positive correlation with the meat production trait(P<0.05); The expression of GHR showed very significant positive correlation with live weight before slaughter, carcass weight, half eviscerated weight, eviscerated weight and breast weight(P<0.01). And the expression of GHR mRNA had significant positive correlation with crureu sweight(P<0.05); the expression of MSTN mRNA showed significant negative correlation with live weight before slaughter, carcass weight, eviscerated weight, breast weight and crureu sweight(P< 0.05). The correlation between the expression of MSTN mRNA and half eviscerated weight was not significant(P>0.05). GHR may play a positive role in the early development of animals, whereas MSTN may play a negative role. |
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