Study On Observation Of Alfalfa Hibernaculum Structure And The Cold Acclimation-specific Gene Under Cold Stress

Alfalfa as an ideal artificial forage grass varieties, which could bring a new method to balance the conflict between grassland and stockbreeding for the sustainable development of Qinghai-Tibet Plateau grassland agriculture. In this thesis, the author explores the situation changes of tissue structure of Qingda No. 1 alfalfa under cold stress through the observation of microstructure; utilizing genetic engineering technology to obtain new strains of cold resistance gene sequence; also identifies and analyzes this gene.As the experiment demonstrates, the more increased the cold stress time, the more tangled the hibernaculum tissue structure of new strain alfalfa. As cold stress time extends, outer skin happens to cracking phenomena, and a particle of the cell walls appear distinctive deformation. Moreover, as the temperature reaches 20 ℃ below zero, the processing time attains 24 hours, the hibernaculum tissue cell nucleus and organelles of some alfalfa starts dissociation phenomenon.In this experiment, the author extracts Qinghai University No.1 alfalfa [Medicago sativa L.cv. Qingda No.1] hibernaculum total RNA, reverse-transcribed into cDNA. According to the CAS sequence design primers for PCR amplification published by Genebank, obtain a 377 bp fragment. Meanwhile, the CAS of the alfalfa contains a 361 bp open reading frame, which shares 96% gene homology with BudCAR6(AF220458.1), shares 95% gene homology with alfalfa pM SACIB CAS15B(L12462.1), and share 90% gene homology with alfalfa CAS15B(KF208641.1). Eventually, the total RNA speculates anden codes 120 amino acids.