Primary Study On Transferring Cold-Resist Genes In Apricot(Prunus Armenica L.)

Growth area of apricot is large in our country. But because of sharp change of the temperature in early spring, the bud, flower and even young fruit will be hurt by the low temperature, and the production of the apricot fruit will be lower, so there is a word that only one year we can harvest the apricot fruit even we culture ten years the apricot tree. Guide outer DNA by pollen tube and with ions are simple, convenience and new methods which without complicated equipment and tissue culture, up these day it has been successfully used in cotton, rice, soybean, wheat, cucumber, tomato and brinjaul etc, and has pioneer a new way of plants' gene engineering. Our research is use apricot (Prunus armenica L.) cv. GoldenSun and Mate transfer outer DNA (afp DNA, BG2 DNA , Pine DNA and Spruce DNA) by pollen tube and with ions. The results is followed.After GoldenSun♀ cross Mate♂ and Mate♂ cross GoldenSun♀ and transferr the outer DNA, effect on development of fruit is not evidently. Rate of survival fruit is affected by cutting flower tube evidently; Content of sugar is equal or higher than the comparison.The rate of survival, stick stem and growth of stick are affected by different genotype evidently. GoldenSun and Mate are suit for transferring the outer DNA by bombarded N ions.Leaves of Golden- sun is thicker than the comparison after been treated, leaves of Mate is thinner the comparison. Color of leaves is natural after been treated with low concentration of N+, color of leaves is bleach slightly after been treated with middle concentration of N+, color of leaves is dark green after been treated with high concentration of N+. Aberrance of the leaves is more evident, some have severe gape at the bottom of the leaves, some have severe gape in the middle of the leaves, some have double nervure and nervure are not symmetry.Size of stoma and leaves are affected by different gene and different segment of DNA.The best sterilize time on young tip explant is 9 minutes with 0.1%HgCl2, the best sterilize time on young buds cultured in culture box is 6 minutes with 0.1%HgCl2.The modified WPM media is the best be propitious to micropropagation of apricot. The optimum concentration of BA is between 0.6—1.0mg/ml, but when the concentration of BA is over 1.0mg/ml, the number of shoots, shoot length and productivity is present fall trend.