The Application Research On Horse Artificial Insemination Technology Of Inner Mongolia

This experiment was conducted to examine the normal semen quality of the 10 chosen stallion which include 4 Thoroughbred horses,2 Budyonnys and 4 Mongolian horses, between 6 to 8 years old with identical feeding conditions and similar weight (500 kg). And another 97 barren mares were chosen to conduct a series of artificial insemination experiments using low dose fertilization method in tubule uterus and in different insemination time, and carried out the mare pregnancy diagnosis after 14 days of insemination. The objectives of this study were to obtain the semen qualities of the three different types of stallion and the optimal insemination time of mares, and ascertain the technologies of diagnosing the early pregnancy of mares through B-ultrasonic scanners. The results are as follows:1. The different types of stallion semen qualities were compared. Among of them, the average ejaculate volume of Budyonnys was significantly higher than that of Thoroughbreds and Mongolian horses (P<0.01) in volume of ejaculation ways; from the aspect of sperm density, the average sperm density of Thoroughbreds was significantly higher than Budyonnys and Mongolian horses (P<0.01); from the point of the total number of sperm, the sperm of Budyonny stallion is the most and the Mongolian horses is secondly. At the same time, the activity of fresh sperm with Mongolian horses was striking higher than that of Thoroughbreds and Budyonnys P<0.01).2. From the aspects of low dose of fertilization in tubule uterus and insemination time, the follicular development of 97 mares achieved diameter of 30mm at first and second season, then using the method of sperm in tubule uterus, and the pregnancy rates reached 55.6%. Furthermore, every time the average sperm reached 2.4ml, and 54 mares were impregnated.3. From the point of Mare cyesiognosis, using the probe with 3.5/5.0 Hz can detect the blastocyst in nearly 14 days after mating and takes sharp images and high resolutions, which showing that high frequency probes suitable for doing the rectal exploration to judging early pregnancy of horses.