Study On Resources Investigation And Quality Evaluation Of The Medicinal Plant Morinda Officinalis How

Morindae Officinalis Radix, the root of Morinda officinalis How, is one of the main medicinal plants in South China. It has the effect of tonifying the kidney yang, strengthening sinew and bone, as well as dispelling wind-dampness. It has been used for impotence and seminal emission, infertility caused by uterine coldness, menstrual irregularities, cold pain in the lower abdomen, painful impediment caused by wind-dampness, limp wilting sinew and bone. The resources of Morinda officinalis How mainly distributed in Guangdong, Guangxi, Fujian and Hainan province. The ecologic studies showed that the shape of cultivate and wild Morinda officinal is How varies a lot. The oligosaccharides in Morinda officinal is How have a lot of pharmacological actions, especially the tetrasccharide to heptaose. The content of anthraquinone and monotropein changes a lot of Morinda officinalis How which comes from different habitats, different ages and different resources. During the investigation of Morinda officinalis How, different places have different cultivation and management. Soil, as the crucial element, influence the quality of Morinda officinalis How. To change the character of soil, fertilizer could helps a lot. Direct control of genetics and indirect effects of external factors influence the growth of herbs, the genetic diversity analysis and identification of Morinda officinalis How using chloroplast genome differences helps resources conservation, locality identification and quality evaluation.The mian studies were summarized as follows:1.Investigation on the resources of Morinda officinalis HowTo investigate different resources of Morinda officinalis How, and lay a foundation for the comprehensive evaluation and screen the excellent medicinal plant of Morinda officinalis How. Field investigations of the resources of Morinda officinalis How in Guangdong, Fujian, Guangxi and Hainan province with the help of literature, then analysis the distribution, botany properties and usage. Different resources of Morinda officinalis How has obvious differences in leaf shape and size, root diameter and character. Guangdong province has a larger cultivated area than any other places;there is a 3333 ha planting area in Deqing county, Zhaoqing city, and Gaoliang town occupied 2000 ha;Gaoliang town has become the famous cultivation bases of Morinda officinalis How;In Guangdong province, it was mainly divided into three types; In Fujian, the planting area were mainly concentrateed in Yingxin and Jichun village,it was also divided into three types; Leaves in Guangxi are smooth; Leaves in Hainan are also smooth. Effective measures should be taken to protect the resources.2. Investigation of Morinda officinalis How oligosaccharides in different habitatsUse the HPLC-ELSD method to determine the four oligosaccharides content of Sucrose,1-Kestose, Nystose and 1F-Fructofuranosylnystose in 32 batches of Morinda officinalis How from different habitats, different ages and different resources, as well as discuss their relationships. Guangdong has the highest level of Sucrose, Nystose and 1F-Fructofuranosylnystose content among Fujian, Guangxi and Hainan, the content of 1-Kestose in Guangdong are similar with Fujian. The germplasm resources of small leaf have the higher content of oligosaccharides than large leaf in Guangdong province. The content of Sucrose,1-Kestose are the highest in the year of 2.5, at the same time, the content of Nystose and 1F-Fructofuranosylnystose are the highest in the year of 4. The content of four Morinda officinalis How oligosaccharides vary from different habitats, different resources and different ages, this research could have helped the quality control of Morinda officinalis How.3. Effect of fertilizers on the growth, isozymes of Morinda officinalis HowA pot experiment was build for studying the influence of different fertilizers including organic, mineral, oligosaccharide and micro-fertilzer on the growth and isozyme actives in Morinda officinal is How. The results showed that the difference of growth among each fertilization was not so notable. The isozyme activities of POD、SOD、EST and MDH were higher in mineral and oligosaccharide when compared to the control; the CYT and MDH isozyme activities were higher in micro-fertilzer. Useing mineral and oligosaccharide ertilizers is a good choice to improve the quality of Morinda officinalis How.4. Genetic diversity ana lysis and identification of Morinda officinalis How using chloroplast genome differencesThe complete chloroplast genome of Morinda officinalis How, an important edible and medicinal plant with great economic value, has been studied in this article. The genome size is 153,398 bp in length, with 38.05% GC content. A pair of inverted repeats (IRs,51,834 bp) are separated by a large single copy region (LSC,83,996 bp) and a small single-copy region (SSC,17,566 bp).The chloroplast genome contains 132 functional genes (103 unique),91 protein-coding genes,33 tRNA genes and 8 rRNA genes. The complete chloroplast genome of Morinda officinalis How in Guangdong province are the same; the complete chloroplast genome in Fujian changes a little when compareds with the samples in Guangdong; the complete chloroplast genome in Guangxi and Hainan changes a lot when compares with the samples in Guangdong. The indels and SNPs among these 15 batches of Morinda officinalis How varies a lot. Analysis of chloroplast in different Morinda officinalis How collections showed that the genetic diversity of different MO germplasms was high.