| Morinda citrifolia, also known as the sea of Morinda officinalis, western countries known as the NONI (Nuo Li), Morinda citrifolia Linn, originated in the South Pacific island of Rubiaceae Morinda tropical medicinal plants.The results show that, Morinda citrifolia is rich in nutritional and functional ingredients, proved to be a broad spectrum of natural plant medicine source is very suitable.In recent years, Morinda citrifolia with treatment and health care effect of special and become the hot spot of research and development, especially in Hainan Island, a unique tropical climate conditions make the Morinda citrifolia industry to become the Hainan new industrial advantage resources, plant Morinda citrifolia has become a new way for local farmers to get rich, Morinda citrifolia planting area has expanded year by year.However, due to its Morinda citrifolia research in our country is late, foundation is weak, the current production appears on some seriously restricted the industrial development factor, such as no cultivars, the planting of Morinda citrifolia is introduced the domestication of wild species, and the origin is too concentrated, narrow genetic types; then the existing cultivars lines are not cold, can not adapt to the north of Hainan and Middle East area short winter low temperature weather conditions and serious injury or even death, makes the Morinda citrifolia planting only in qiongnan area, restricted the development of the industry.At the same time information retrieval results, the domestic and foreign research in Morinda citrifolia mainly focus on effective components and health care and the treatment effect and so on, so far, the research reports on Morinda citrifolia germplasm resources is still blank. Therefore, this project markers and molecular morphological markers for genetic diversity research on germplasm resources of Morinda citrifolia, through the study of morphological characteristics, karyotype of Morinda citrifolia, morphological diversity and SRAP molecular markers in genetic diversityã€analysis and comparison, determine the characteristics of the germplasm, the chromosome number and karyotype, relationship genetic diversity and genetic, and finally establish the genetic diversity of germplasm resources database of Morinda citrifolial.The results of this study are as follows:1ã€Research has obtained the Morinda citrifolia root, stem, leaf, flower, fruit and seed morphological characteristics of botanical description result.First proposed the Morinda citrifolia fruit as the fruit and non fruit an aggregate. 2ã€Morinda citrifolia morphological cluster analysis showed that,78accessions were clustered into six categories, respectively is:the Wan Wei No.2, Singapore, the Wan Wei No.1, Tahiti, Hainan wild populations and Paracel Islands populations.The Wan Wei No.1populations and Tahiti in the morphological genetic distance group0.3ã€Methods the best production Morinda citrifolia chromosome is:the1.0-1.5cm long leaves, in0.4%colchicine pretreatment of4h, the "1.5%cellulose enzyme pectinase enzyme mixture of-1.5%fiber"1H "treatment can obtain the best effect of dissociation, the modified magenta dye, chromosome drop method can obtain the ideal.4ã€The chromosome number of Morinda citrifolia cell is16,2n=16, the karyotype of Morinda citrifolia is2n=16=10m+4T+2t.5ã€SRAP molecular marker clustering analysis showed that, at the genetic distance of0.86threshold,78copies of Morinda citrifolia germplasm materials can be divided into six parts, respectively is:1the WanWei No.2,2in Singapore,3Tahitian,4the WanWei No.1,5Hainan native,6Paracel Islands. The clustering result with morphological polymorphism clustering results.6ã€Through correlation test, on the morphology of clustering and SRAP molecular marker clustering results, the correlation between the two reached a very significant level, results show that the cluster with two markers is consistent. |
