High Efficient Regeneration System Establishment Of Artemisia Selengensis Turcz

Artemisia Selengensis Turcz was Artemisia (Compositae), which was delicious and nutritious, and had very highly, edible and medicinal value. The traditional breeding mode of Artemisia Selengensis was excavating underground rhizomes, which severely damaged the ecological environment of origin place and was harmful to wild germplasm protection of Artemisia Selengensis. Additionally, successively planting caused the frequent diseases and insect pests, so the yield and quality of Artemisia Selengensis decreased. Using wild Artemisia Selengensis collected from Hulun Buir in Inner Mongolia as test material, selecting stems with growing points as explants, this study inducted proliferation of growing points and rooting to establish high efficient regeneration system of Artemisia Selengensis. High efficient regeneration system establishment of will achieve rapid production of virus-free seedlings and improve the yield and quality of Artemisia Selengensis in order to provide high quality green vegetables for the market. The test studied the effects of 0.1% mercuric chloride sterilization time and different combinations of 6-BA and NAA concentration on initiation culturing of Artemisia Selengensis, the effects of different combinations of 6-BA and NAA concentration on primary culturing of Artemisia Selengensis, the effects of plant growth regulator types and ratios on subculturing of Artemisia Selengensis, and the effects of auxin types or concentrations and culture medium types on rooting culturing of Artemisia Selengensis. Finally, established the high efficient regeneration system of Artemisia Selengensis. The results of the study are as follows:1. Initiation cultureUsing stem as the test material, disinfect 8min with 0.1% mercuric chloride was the most appropriate disinfection time, the survival rate of explant was 20%. The most appropriate medium of initiation culturing was MS+6-BAo.5mg·L-1+NAA0.02mg·L-1, the induction rate was 100%, the proliferation multiple was 4.37.2. Primary cultureMS+6-BA0.05mg·L-1+NAA 0.01mg·L-1 was the most appropriate medium of primary culturing, the induction rate was 100%, the proliferation multiple was 14.27.3. SubcultureIn the process of subculturing, the most appropriate cytokinin type was 6-BA, the most appropriate auxin type was IBA. MS+6-BA0.5mg·L-1+IBA0.4mg·L-1 was the most appropriate medium of subculturing, the induction rate was 100%, the proliferation multiple was 23.08.4. Rooting cultureThe most appropriate auxin type to induct rooting was NAA, the most appropriate NAA concentration to induct rooting was 0.1mg·L-1. MS+NAA0.1mg·L-1 was the most appropriate medium of rooting culturing, the rooting rate was 100%, the root number was 42.9 per test-tube plantlet, the average root length was 5.2cm.