Study On Tissue Culture And Propagation Technique Of Oil Peony

Different explants of Paeonia ostii ‘Feng Dan’ were used to study its disinfection, browning control, proliferation induction, rooting and other issues. The results were as follows:1. 75% alcohol disinfection of 30 S and 0.1% mercuric chloride disinfection of 8min was a better treatment on buds of Paeonia ostii ‘Feng Dan’; For peony seeds, with 75% alcohol disinfection 50 s, or with 0.1% sodium hypochlorite for 5 minutes is a better treatment.2. Browning rate could be controlled under the cultivation combined chilling with 3 mg/L AgNO3..3. The most appropriate medium for explants inducing was the medium of MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L, after 7 days of culture buds began to sprout, induction rate could reach 82.32%; the most suitable medium for propagation was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + PIC(Picloram) 1.0 mg/L; The best rooting medium was 1/2MS + CaCl2(220 mg/L) + IBA 3.0 mg/L + NAA 0.5 mg/L.4. 2.0 mg/L GA3 + 0.1 mg/L NAA is a better induction treatment for Peony embryo. Cotyledons and radicle length of time to a time significantly advance, these embryo growth healthy.5. Different concentrations of 6-BA and NAA combinations for three explants conducted callus, we found that embryo of "Feng Dan" Peony callus induction better, stem and leaf followed; 3.0 mg/L 6-BA + 0.6 mg/L NAA, inducing more suitable species embryos,can has better induce embryonic callus;6. Different concentrations of 6-BA and 2,4-D combinations for three explants conducted callus. On the same 6-BA and 2,4-D concentration of the culture medium, embryo, stem and leaf callus induction rate were not significant. And when 6-BA was 3.0 mg/L and 2,4-D was 1.5 mg/L, induction rates of these three callus were 93.14%, 81.57%, 79.40%. But callus formed mostly white loose slug tissue, which were not cultured differentiation lately.