Reserch Of Typical Vegetation Soil Microbial Diversity And Actinomycetes Resource Of Taibai Mountain

Qin Mountains is a natural geographic and climatic boundary between North and South China. Taibai Mountain the main peak of Qin Mountains, huge mountain gap created a unique vertical climatic zones, vegetation zones and soil types change accordingly, possessing diverse biological resources. The present paper explored the method of actinomycetes isolation through five medias by using soil samples of diffrent plant types as material.Phylogeny method was applied to study the microbiome distribution and composition of actinomycetes around Taibai mountain using strains isolated from soil samples. We also studied bacteria diversity of Taibai mountain by using high-throughput sequencing technology.The main results are as follows:1 PH content on south slope range from 5.68 to 6.41, while 5.07 to 6.58 on north slope,there was a larger amplituder on north slope than that on south slope, the lowest pH was 5.07.Soil organic matter content had relatively greater range, maximum was 77.8 g/kg while minimum was 11.7 g/kg. The highest content of total nitrogen on south slope was 5.35 g/kg,2.81 g/kg on north slope, meaning total nitrogen content was higher on south slope; The content of nitrate and ammonium nitrogen were consistent on both slopes; Available phosphorus content of red skins birch was relatively high, the highest value was 7.15 mg/kg,the lowest content was 2.69 mg/kg in alpine meadow soil; Available potassium content basically the same on both sides. The soil nutrient of Taibai mountain presents a trend of relatively high content in mid-elevation plant types, such as Abies fargesii, little content in low altitude and high altitude, such as Quercus aliena var.acuteserrata and Alpine meadow,which meaned soil nutrient presented a trend of rising then reducing with altitude increasing.As a whole, soil nutrient is relatively rich on south slope than that on north slope.2 Analysis of actinomycetes number and variety on different soils of plant types showed that modification glycerin- asparagine media was apparently higher than that from others,with the quantity of 258×104 cfu/g dry soil, 35 species kinds. No.1 media showed a better separation effect on the south slope, which meaned more suitable for the separation of the south slope of different forest soil actinomyces. There are obvious advantages of R2 A media while isolating mid-elevation forest type. Actinomycetes separation effect on trehalose-proline medium was the worst, with the quantity of 7.0 × 104cfu/g dry soil, only 4 species kinds. The elevation forest types, such as Redskins birch, Birch on north slope, Birch on southern slope, the quantity of isolated actinomycetes were significantly higher than other types of woodland, a the minimum number of actinomycetes separation on alpine meadows.3 The 16 S rDNA was sequenced of 427 actinomycetes strains isolated from Taibai mountain soil samples and blasted in the database of Ez Biocloud. The results showed that the isolated strains could be classified into 89 species and 23 genus, which implied that there were abundant of actinomycetes resource in Taibai mountain. The dominant bacterial community was Streptomyces, which was 88.06% of the strains, S.enissocaesilis, S.cirratus and S.nojiriensis were dominant species. The remaining 22 genus excet of Streptomyces,dominant bacterial community were Nocardia, Kitasatospora, Renibacterium, Arthorobacter.4 There was a significant difference of actinomyces genus on both slopes of Taibai mountain. In the 23 generas identified, the both slopes shared 11 genus, north slope had 7uniqe genus, respectively Overskovia, Acinetobacter, Staphylococcus, Brevibacillus,Flavobacterium, Kribbella, Pseudomonas. North slope had 5 unique genus, respectively Aggregatibacter, Methylophilus, Micromonospora, Novosphingobium, Pseudoxanthomonas.Different vegetation diversity analysis showed each of vegetation had its unique genus, which meaned there was a significantly difference in vegetation soils.5 Good’s coverage of high-throughput sequencing was 98.1-99.4% under the condition of similarity of 0.97, illustrated the sequencing results can accurately reflect the soil microbial diversity. The dominant phylums were Proteobacteria, Acidobacteria, Actinobacteria,Chloroflexi, Nitrospirae, Bacteroidetes, Gemmatimonadetes, Firmicutes, Verrucomicrobia,Fibrobacteres, Candidates-division-TM7, Planctomycetes, Candidates-division-WS3. The abundance of Actinobacteria in different soil samples existed discrepancy. Quercus liaotungensis on north slope, larix chinensis on both slopes showed a relative high abundance,more than 20%, alpine meadow, on the highest elevation, showed a general abundance. The relative abundance of Actinobacteria presented a trend of decreasing than rising, which was the same with the distribution of isolated actinomycetes.