Identification And Functional Analysis Of BZIP Transcription Factor Genes Related To UPR In Wheat

As an important food crop, wheat play an integral role in the national production and life. Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most serious air-borne fungus diseases in wheat worldwide. According to the statistics,the huge outbreaks of stripe rust often resulted in a significant decline in wheat production. It has been proved that selection and rational use resistant varieties is the safest and most efficient way in controlling stripe rust. The cloning of resistance genes will greatly promote insights into the mechanism of host-pathogen interaction and provide the diversity of options available for durable resistance.As the main synthesis and processing sites of resistance associated protein (such as PR proteins and PRRs), endoplasmic reticulum plays a central role in regulating the immune response of the plant.The destruction of its function is one of the main factors of fungal infection of the host cell.Two arms of the UPR signaling pathway have been described in plants:one that involves two ER membrane-associated transcription factors (bZIP17 and bZIP28) and another that involves a dual protein kinase (RNA-splicing factor IRE1) and its target RNA(bZIP60).In this study,three bZIP transcription factor genes have been cloned and named TabZIP60, TabZiP28, TabZIPl 7. Using CYR32 and wheat cultivars (NB-1 and Yangmai 158) to constitute Compatible interaction and Incompatible interaction to analysis their expression.TabZIP60 gene has 925bp open reading frame,encoding 244 amino acids. Subcellular localization experiment revealed that it was located in the nucleus and the transcriptional activation experiment proved that it has transcription activity. Phylogenetic analysis indicated that it has the closest evolutionary relationship with barley, followed by corn, rice and Arabidopsis. Quantitative PCR results revealed that TabZIP60 has bimodal expression trends in the incompatible interaction,combining with resistance gene expression profiles and strips secondary infection process infer that it may be involved in the resistance gene induced signaling pathways.TabZIP28 gene has 1188bp open reading frame, encoding 395 amino acids.The expression level in the compatible interaction is not obvious,while it reaches its maximum at 24h in the incompatible interaction. Combining with characteristic analysis of endoplasmic reticulum stress conclude that this gene participates in the early stage of defense response.TabZIP17 gene has a 1764bp complete reading frame, encoding 587 amino acids.The changes of expression level are not obvious both in the compatible interaction and incompatible interaction.Thus, we speculate that it may not participate in the inducted resistance response of resistance gene.