| Stripe rust is a devastating aeroborne disease in wheat production worldwide. Stripe rust, which is caused by Puccinia striiformis f. sp. tritici, threaten wheat production safety with the charactors of wide in influnce and easy to epidemic. The appropriate use of resistance cultivars offers the most economic, safe, and environmental friendly way to control the disease. It is important for application of plant disease resistance to research the mechanism of resistance to stripe rust and interaction between wheat and stripe rust fungi.Proper growth and development function depend on accurate and complete gene expression. Gene regulation depend on the protein-DNA interaction or protein-protein interaction. MADS box protein belong to binding transcription factors which specific regulate combination with DNA and play pleiotropic functions in growth and development in fungi and plant. Host-pathogen interactions analysis depends on the determination of their gene products, as well as signal reception and transmission of molecular biology technologies. In this experiment, We screen the MADS-box gene PsMcm1 participated or related to stripe rust’s growth or pathogenic in the genomic level. PsMcm1 was up-regulated in the protophase of infection. The encoding protein PsMcm1 has interaction with its upstream protein PsSte12 and located in cell nucleus. Pathogenicity of Magnaporthe oryzae was recovered partially when completing PsMcm1 to mutant Momcm1. Sexual reproduction of Saccharomyces cerevisiae was recovered when completing PsMcm1 and PsSte12 to mutants Scmcm1 and Scste12. The growth and development of rust fungi and the uredospore production were decresed significantly in PsMcm1 and PsSte12 instantaneous silenceing plants. Overall, our data indicate that PsMcm1 may plays a critical role in the regulation of growth and development, sexual reproduction and pathogenesis in rust fungi in cooperation with cofactor PsSte12. |
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