| Agricultural adjuvants can affect the efficacy of pesticide in the field, and also change its behavior in the environment, such as soil, plant. Neonicotinoid insecticides are famous for their high toxicities to honeybee, and acetamiprid with relative low acute toxicity to honeybee among this group of insecticides. In this project, three commonly used pesticide adjuvants, N-methylpyrrolidone (NMP), Silwet L-77,Triton X-100 will be studied their effects on the acute toxicity to honeybee in the laboratory, as well as effects on the mortality, flight intensity,behavior, brood and colony development of honeybees under semi-field conditions. Objectives of this research is to understand the effect of agricultural adjuvants on the honeybee toxicity of acetamiprid, which will help us to take measures to manage the environmental risk of pesticide during formulation processing.The results indicated that 3 types of pesticide adjuvants have no acute toxicity to honeybee in significant, as well as effects on the acute toxicity to honeybee of acetamiprid by feed. However, increased contact toxicity to honeybee of acetamiprid in significant. In semi-field test,5% acetamiprid SL(soluble concentrate) mixed with3different pesticide adjuvants in respectively, contrast to the purewater and dimethoute as positive CK, the death rate, flying ability, pollen collection, and population and its development (egg, larva,pupa) were affected in significant. In the groupT2(acetamiprid+Silwet L-77) test, the death rate of honeybee were higher than CK in significant at DAA-4, and the population number were lower than positive control at DAA-28. In all three tests, the mortality, population number and development of honeybees were different in significant.In conclusion, the pesticide adjuvant Silwet L-77 increased the acetamiprid risk on environment in potential particularly in honeybees. |