| This study was to conducted to investigate the influence of organic trace minerals (oTM) on performance, egg quality and serum immunity and antioxidation activity of laying hens at reduced levels. oTM was the combination of Small peptides chelated iron, copper, manganese, zinc and nano-selenium. A total of 225 healthy Hy-Line Brown Layers (35 weeks of age) were randomly divided into 5 groups with 5 replicates per groups and 9 hens per replicates. Hens in the control groups were fed the basal diet supplemented with inorganic trace minerals (iTM),i.e., Fe, Cu, Mn, Zn, Se at 75,6,60,60,0.3 mg·kg-1. In another fore diets, the basal diet was supplemented with the combination of small peptides chelated iron, copper, manganese, zinc and nano-selenium at 70,60,50,40% levels of iTM. i.e.,70% oTM treat,60% oTM treat,50% oTM treat,40% oTM treat. The adjustment period lasted for 7days,and the experimental period lasted for 42 days. Hens’ production quotas were recorded, Egg samples were collected to measure egg quality at 14,28,42d, serum samples were collected to measure serum immunity and antioxidation activity.Metabolic trial was after feeding trial. Results showed as follows:(1) The performance was not affected by reducing the supplementation of oTM concentration in the diets. Similarly, compared with the control group, the feed to egg ratio in 60%oTM was decreased (P<0.05) and lying rat was increased (P<0.05).(2)There was no significant difference in egg quality between the treatment groups and control group. It concluded that oTM at reduced level could keep the normal performance.(3) Groups supplement different levels of oTM could significantly increased the Ca content of serum by 4.94%(P<0.01)、45.24%(P<0.01)、26.73%(P<0.01)、32.90%(P<0.01); only 70% oTM and 60% oTM have 25.34% increased the P content of serum by(P<0.05)、39.04% (P<0.01);There was no significant difference in Glu、ALP、UA, but the ALP in the oTM was higher than iTM; Dietary oTM or iTM had no significant effect on the metabolism of lipid.(4) The serum GSH-Px activity was significantly increased (P<0.05), but other oTM diets have decreased the serum GSH-Px activity(P<0.05);Each group is not different on the T-SOD, but all oTM diets have increased T-AOC (P<0.01); Only 70% oTM and 60% oTM have decreased the content of MDA (P<0.05).(5) Two kinds of trace minerals had no significant effects on the content of serum IgA.Serum IgM levels tended to increase,40% oTM could significantly increased IgM levels (P<0.05),but 40% and 50% oTM decreased IgG levels. Total protein (TP) and albumin (ALB) in oTM groups were significantly increased.(6) There was no significant difference on the content of Cu、Mn、Fe in egg yolk, groups supplement with 60% oTM significantly increased Zn levels (P<0.05) by 22.92%, although 50% and 40% oTM increased the Zn levels, but reduced the Se content. Except the Fe in manure, Cu、Mn、Zn content in manure have been markedly (P<0.01) reduced as the level of oTM increased, the content of Cu was reduced by 49.47%(P<0.01)、57.29%(P<0.01)、 62.16%(P<0.01)、64.42%(P<0.01), the content of Mn was reduced by 28.32%(P<0.01) 36.91%(P<0.01)、40.00%(P<0.01)、42.26%(P<0.01); the content of Zn was reduced by 22.33%(P<0.01)、24.71%(P<0.01)、26.56%(P<0.01)、28.29%(P<0.01).The dietary supplementation of trace minerals can be reduced greatly when supplemented with the the combination of Small peptides chelated iron, copper, manganese, zinc and nano-selenium without affecting performance and egg quality status in laying hens, and it could improve immune and antioxidant functions,and the absorption of Ca and P, while it has no significant difference on the metabolism of lipid at the level of 70% or 60% oTM. Therefore, it is concluded that 60% oTM was the best diets for keeping the normal physiological function of laying hens. |
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