Effects Of Total Phenols From Magnolia Officinalis On The Growth Performance, Antioxidant Ability And Intestinal Function Of Yellow Broilers

The effects of total phenols from Magnolia officinalis on the growth performance, antioxidant ability and intestinal function of yellow broiles were investigated.360 yellow broilers (cocks) of age of 1 day were divided into 5 treatments, with the antibiotic group,0 mg/kg,50 mg/kg,150 mg/kg,250 mg/kg group with total phenols from Magnolia officinalis. There were 6 replicates per treatment and 12 birds per replicate. The trial was conducted for 56 days. Serum, liver, bowel, breast and leg muscle samples were collected in the day of 28 day old and 56 day old respectively. Serum antioxidant indicators, meat quality, intestinal morphology and intestinal microflora were detected. The results showed:(1) In the post-test, the average daily feed intake of 50 mg/kg group was significantly higher than other groups without 250 mg/kg group (P<0.01). However, the feed conversion efficiency of 150 mg/kg group was significant higher than 50 mg/kg group (P<0.01), but no difference was obsered between 150 mg/kg group and others. In the whole period of experiment, the average daily feed intake of trial groups were superior to the antibiotic group (P<0.01), and a quadratic improvement relationship was fund in the feed conversion efficiency of trial groups.(2) At the age of 28 days, the activity of T-SOD and CAT in serum of 150 mg/kg group were slightly similar to the antibiotic group, and the activity of T-SOD was significantly higher than other groups (P<0.05), CAT activity was significantly higher than 0 mg/kg group(.P<0.05). The level of 8-Hydroxy-2-deoxyguanosine in serum of all groups were significantly lower than 0 mg/kg group (P<0.05). In 56 days, The level of serum protein carbonylation of 50 mg/kg group was significantly lower than 0 mg/kg group (P<0.05).(3) In 28 days, the villus height of ileum of each group was higher than the antibiotic group (P<0.05), and the crypt depth of duodenal of 150 mg/kg group was significantly lower than others (P0<.05).The crypt depth of ileum of 250 mg/kg group was lower than the antibiotic group, while the antibiotic group and 150 mg/kg group were significantly lower than others in 56d (P<0.05).(4) In 56d, pH45min and pH24h of breast muscle of 50 mg/kg and 150 mg/kg groups were higher than others (P<0.05). a* value of breast and leg muscle of 150 mg/kg group were significantly higher than others except the antibiotic group of the leg muscle (P<0.05). Water drip loss in breast muscle of 150 mg/kg group was significantly lower than other groups without 250 mg/kg group in 24 h, and its water drip loss was significantly lower than 0 mg/kg group in 48 h, while its water loss rate was significantly lower than others. Water drip loss (48 h) in leg muscle of 150 mg/kg group was significantly lower than 0 mg/kg group and 50 mg/kg group (P<0.05).(5) The cecum of 56-day-old yellow broilers was sequenced with 16s rDNA. The results showed that 1230643 raw tags and 1230039 clean tags were got in the study, in addition, the average length was 253 bp. We observed high microbial diversity in the cecum. At Phylum level, Firmicute, Proteobacteria, Bacteroidetes was the most three phylum in the study. The relative abundance of Firmicute at phylum level of 50 mg/kg group was higher than other groups, while the antibiotic group,50 mg/kg and 150 mg/kg were higher than others of the relative abundance of Proteobacteria.All the results indicated that total phenols from Magnolol officinalis can improve the growth performance, antioxidant function, and intestinal function of yellow broilers. In addition, The dose of 150 mg/kg total phenols from Magnolol officinalis was the better select.