Effects Of Magnolol On Growth Performance,Meat Quality,Antioxidant Capacity,and Immune Function In Fast Yellow-Feathered Broilers

This experiment was conducted to study the effects of Magnolol(MAG)on growth performance,meat quality,immune function,and antioxidant capacity in yellow-feathered broilers.The experiment was used to select 360 1-day-old healthy male yellow-feather broilers with similar body weight and randomly divided into 5 treatment groups with 6 replicates in each group and 12 chickens in each replicate.Broilers in control group were fed a basal diet,and the others in experimental groups were fed the basal diets supplemented with 100,200,300 and 400 mg/kg magnolol,respectively.The experiment lasted for 51 days.Research indicates:(1)Effects of magnolol on growth performance in yellow-feather broilersAt the end of the experiment,compared with the control group,the MAG200 and MAG300 groups significantly increased the final weight of the yellow feather broilers(P<0.05).During day 1 to 28,dietary treatments did not affect ADFI,ADG,or F/G(P>0.05).During day 29 to 51,the ADFI of the MAG300 group was significantly higher than the control group(P<0.05),and the ADG of the MAG200 and MAG300 groups was significantly higher than the control group(P<0.05).From the perspective of the entire growth period,compared with the control group,the ADG of the MAG200 and MAG300 groups was significantly increased(P<0.05),and the F/G was reduced(P<0.05).It shows that magnolol does not affect the feed intake of broilers,but reduces the F/G by increasing the average daily gain of broilers,and improves the growth performance of broilers,and the effect of growth performance in MAG200 and MAG300 groups are better.(2)Effects of magnolol on carcass performance and meat quality of the breast muscle in yellow-feather broilersCompared with the control group,MAG200 and MAG300 groups significantly increased the breast muscle rate.The supplementation of magnolol had no significant effect on the dressig percentage,full eviscerated,leg muscle rate,and abdominal fat rate(P>0.05).The MAG300 group significantly improved the a* value(P<0.05).The expressible moisture of MAG100,MAG200,and MAG300 groups was significantly lower than the control group(P<0.05).Compared with the control group,each group in the experiment can reduce the breast muscle shear force(P<0.05).The supplementation of magnolol had no significant effect on p H24 h,L* value and b* value(P>0.05).It shows that supplementation of magnolol to feed has no effect on the carcass performance in yellow feather broilers,but it can improve the quality of the breast muscle,which is mainly reflected in improving muscle tenderness and water holding capacity,and increasing the redness of meat color.(3)Effects of magnolol on immune function in yellow-feather broilersAt 28 days of age,adding 100 mg/kg magnolol to feed had no significant effect on the serum Ig G,Ig M,and IgA contents of yellow feather broilers(P>0.05).The serum Ig G,Ig M and IgA levels of yellow feather broilers in the MAG200,MAG300,and MAG400 groups were higher than those in the control group(P<0.05).Compared with the control group,the MAG300 and MAG400 group significantly increased the jejunum IL-10 and IL-4 content(P<0.05),and the MAG200,MAG300,and MAG400 groups jejunum IL-2 content significantly increased(P<0.05),and each experimental group can significantly increase the content of IL-12 and IFN-γ in the jejunum(P<0.05).The supplementation of magnolol to feed had no significant effect on colonic IL-10 and IL-2,but there was a tendency to increase(P>0.05).Compared with the control group,the MAG200,MAG300,and MAG400 groups can significantly increase the contents of IL-4,IL-2,and IFN-γ in the colon(P<0.05).At 51 days of age,supplementation of 200,300,400 mg/kg magnolol can significantly increase the serum Ig G and IgA content of yellow feather broilers(P<0.05).Compared with the control group,the serum Ig M in the yellow feather broilers in the MAG300 and MAG400 groups were significantly increased(P<0.05).The levels of IL-10 and IL-4 in the jejunum of the 200,300,and 400 mg/kg magnolol groups were significantly higher than those of the control group and the 100 mg/kg magnolol group(P<0.05).Compared with the control group,the test group’s jejunum.The contents of IL-2,IL-12 and IFN-γ were significantly increased(P<0.05).The contents of IL-10 and IFN-γ in the colon were significantly increased in the MAG200,MAG300,and MAG400 groups(P<0.05),and the IL-4 content was the highest in the MAG300 and MAG400 groups(P<0.05)),the levels of IL-2 and IL-12 in the experiment group were higher than those in the control group(P<0.05).The s IgA in the intestines of 28 days of age and 51 days of age yellow feather broilers,regardless of jejunum and colon,was significantly higher in the test group than in the control group(P<0.05).It shows that magnolol has a positive effect on intestinal immune function.(4)Effect of magnolol on Nrf2 Signaling Pathway–Related Genes of the breast muscle in yellow-feather broilersAt 28 days of age,compared with the control group,the MAG200 and MAG400 groups significantly increased the expression of Nrf2,NQO1,HO-1,GCLC,and SOD in the breast muscle(P<0.05).The MAG100 and MAG400 groups significantly increased the expression of GCLM in the breast muscle of yellow feather broilers(P<0.05).At 51 days of age,compared with the control group,the MAG100 and MAG200 groups significantly increased the expression of GST and SOD in the breast muscle of yellow feather broilers(P<0.05),and the MAG300 group significantly increased the expression of Nrf2,NQO1,HO-1,GST,GCLM,and SOD in the breast muscle of yellow feather broilers(P<0.05).(5)Effect of magnolol on gut microbiota in yellow-feather broilersThe supplementation of magnolol to feed significantly reduced the Chao1 index of yellow-feather broilers at 28 days of age(P<0.05),indicating that magnolol would reduce the abundance of the caecal flora of yellow-feather broilers in the early growth period;Magnolol had no significant effect on the cecal flora diversity and abundance of yellow feather broilers at 28 days and 51 days(P>0.05).LEf Se analysis found that compared with the control group,the experimental group reduced the abundance of Hydrogenophilales,Peptostreptococcaceae,Clostridium,and Thiobacillus,and increased the abundance of Fusobacteria,Chloroplast,Lactobacillales,Alipis,Rikenella Sphingomonadales,Veillonellaceae.At 51 days of age,LEf Se analysis showed that compared with the control group,the experimental group decreased the abundance of Deltaproteobacteria,Pseudomonadales,Hydrogenophilales,and Thiobacillus,and increased the abundance of Fusobacteria,Odoribacter,Porphyromonadaceae,Faecalibacterium,Succinispira,Megamonas,and megasphaera.