| Rabbits’growth rate and weight were regulated by the energy metabolism. Thus, genes involved in energy metabolism were possible associated with rabbit growth traits. The main funcation of the ADRB3 gene was to regulated the body fat metabolism and energy metabolism. A total of 304 unrelated individuals from three meat rabbit breeds:New Zealand,Ira and Tianfu black were used in this study. ADRB3 as candidate gene, genetic variation of the coding sequence were identified and the effect of this gene on growth were evaluated.In addition, comparation and analysis of three varieties of rabbit on growth traits was done in this study.The results were as follows:1.There was not significant difference among three varieties of rabbit on 35,70 and 84 days body weight. However, multiple comparison showed that Tianfu black rabbit had a greater 70 days body weight than Iraq rabbit (P<0.05), and had a greater 84 days body weight than the other two rabbits (P<0.05); New Zealand rabbit had a greater 28 days body weight than Iraq rabbit (P<0.05), and had a greater 35 days body weight than the other two rabbits; New Zealand rabbit also had a greater 70 and 80 days body weight than Iraq rabbit. Although there was not significant difference among three varieties of rabbit on the average daily gain(ADG), but the Tianfu black rabbit had a greater ADG than the other two rabbtis; New Zealand rabbit had a greater ADG than Iraq rabbit.2. A total of 4 variants in ADRB3 gene were detected in exon 1, and all of these SNPs were synonymous mutations:c.36 C>T,c.144 G>T,c.157 C>T and c.489 C>T. The results of protein domain prediction showed that three of these SNPs were located in Solcar domain. And the c.144 G>T and c.157 C>T loci were located in the first transmembrane helix domain; c.489 C>T loci was located in the fourth transmembrane helix domain. Linkage disequilibrium analysis showed that c.157 C>T loci and the remaining two SNPs in very strong degree of linkage (r2>0.75), which suggested that this locus had similar genetic effects with remaining two SNPs. We finally chose c.157 C>T for further study.3.The c.157 C>T locus possessed moderate genetic diversity (0.25<PIC<0.50) in three rabbit breeds; χ2 showed that this locus in Hardy-Weinberg equilibrium in New Zealand,Ira and Tianfu black rabbits (P>0.05).4.The association study showed that c.157 C>T has a significant effect on 70 days and 84 days body weight and average daily gain(ADG) in New Zealand, Ira and Tianfu black rabbits (P<0.05). And individuals with CC genotype had a greater 70 days body weight,84 days body weight, ADG than those with TT genotype (P< 0.05).Moreover, individuals with CC genotype had a greater 28 days body weight and 35 days body weight than those with CT and TT genotype. |
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