Research On Protective Effect And Mechanism Of Shenqin Compound To Canine Parvovirus In Cardiac Tissue

Canine parvovirus disease is an acute infectious disease caused by canine parvovirus (Canine parvo-virus, CPV) infection puppies, severe vomiting, hemorrhagic enteritis and white blood cells was significantly reduced myocarditis as the main feature, the high incidence of infection strong, high mortality, it is against the dog industry in China one of the most serious infectious diseases can cause serious economic losses. Veterinary theoretical analysis, canine parvovirus is a hot and humid Xie Li, Yi Qing and hot and humid to stop diarrhea. For such symptoms should raise the heart stasis, clearing heat and detoxification. In accordance with the principles of dialectical theory of Chinese medicine and clinical dialectical self Compound Senate Baicalensis prevention, Fangzhong Dan parameters stasis, strengthen myocardial contractility improvement in cardiac function, and promote tissue repair and the role of Baicalin, matrine heat dampness, the Lord used to the diuretic heat, damp, Xie Li, hot shower with antibacterial antipyretic pharmacological effects. Used for the prevention and treatment of canine parvovirus, in order to lay the foundation for the mechanism and clinical application of Shenqin compound control canine parvovirus.First, to explore Shenqin compound artificially infected with the efficacy of myocarditis parvovirus dogs, dogs were divided into blank control group, model group, positive control group, the compound parameter baicalensis group, canine parvovirus vaccination to establish animal models, compound parameters baicalensis prevention of canine parvovirus, and application of bio-signal acquisition system ECG measured by test animals, heart rate, electrocardiogram, take heart embedded in paraffin, sliced, microscopic examination. The test results showed that 3 days after inoculation, the model group dogs heart rate and electrocardiogram abnormal rate increases, severe myocardial damage; compound parameter baicalensis group of dogs heart rate remained normal, abnormal ECG was significantly lower than the model group dogs, the myocardial tissue with the control group The difference was not significant. Experimental dogs infected with the virus 5 days, blank dogs in the control group does not appear to death, the model group of dogs the mortality rate was 100%, Shenqin compound group protection rate of 80%, the positive control group 70% protection rate. Show that Shenqin compound can be effective in preventing myocarditis-type canine parvovirus disease.Second, the study Shenqin compound protective effect of artificially infected myocarditis parvovirus dogs heart and lung. By optical microscopy, transmission electron microscopy observation of the dogs, the heart and lung pathological changes of cardiac tissue structure of Shenqin compound dogs, canine parvovirus, the ultrastructure of The test results show that with the control group, model group of canine cardiac tissue lesions and inflammatory cell infiltration, electron microscope, cell shrinkage, nuclear condensation, deeply stained, swollen mitochondria with cavitation; Compared with model group, Shenqin compound against myocardial mild tissue lesions, ultrastructure did not change significantly; study showed that the compound parameter baicalensis protection of the heart to restore the obvious, and allows myocardial thickening, and enhanced cardiac activity. myocardial cells were short cylindrical, abundant cytoplasm and red dye, the nucleus oval, at the central of the cell; better restore protective effect of damage to the heart.Third, explore the compound parameter baicalensis antioxidant effects of neonatal rat cardiomyocytes, determination of the SOD, the amount of MDA in the lysates of neonatal rat cardiomyocytes through the test to compare Shenqin compound prevent myocardial cells from the damage of canine parvovirus; results the virus control group compared with normal controls the difference was significant (P<0.01) SOD in the cell lysate to reduce the MDA content increased; compound parameter Baicalensis, dose group and virus control group compared to the significant difference (P<0.01) cell lysis. SOD increased, MDA lower fluid. Conclusion:Compound parameters baicalensis can effectively protect the neonatal rat myocardial cells from the damage of canine parvovirus.Fourth, the study Shenqin compound induced cardiomyocyte apoptosis and related gene expression in canine parvovirus. Methods:The dogs were divided into blank control group, model group, positive group, the compound parameter baicalensis prevention group inoculation to establish the animal model, canine parvovirus, the compound Senate Baicalensis prevention canine parvovirus virus disease, and then sampled, using fluorescence quantitative method to detect Bcl 2 and Bax gene mRNA expression. Results:The comparison with the blank control group, model group Myocardial cell expression of Bcl-2 gene down Bax expression increased. Compared with model group, the compound parameters the baicalensis prevention group myocardial Bax gene downregulated Bcl-2 gene upregulation. Conclusion:Shenqin compound may be apoptosis by promoting cell proliferation and recovery of myocardial regulation of Bcl-2 and Bax gene expression and inhibition of myocardial cells caused by canine parvovirus parvovirus dogs from the myocardial tissue structure to protect and promote recovery role.In summary, Shenqin compound can effectively prevent canine parvovirus, dogs, heart and lung tissues and organs have a protective effect on tissue damage caused by parvovirus repair; The mechanism may be by regulating the Bcl-2 and Bax mRNA expression increased Bcl-2/Bax ratio. Had increased the Bcl-2/Bax ratio may reduce the release of Cyto-C, change redox state, lowered oxidative stress in the role, and to reduce the signal transduction initiated by reactive oxygen species, thus inhibiting Caspase apoptotic cascade pathway, inhibition of cell apoptosis. The exact mechanism has yet to be through further study.