Preliminary Screening Of Excellent Hybrid Strains Of Pleurotus Eryngii Var. Tuoliensis

The physiological, biochemical and cultural characteristics of 5 Pleurotus eryngii var. tuoliensis strains were analysed by antagonistic test, esterase isozyme analysis and fruiting test. Using “zhong nong 1” and “zhong nong chi bao” with complementary advantages were as crossing parents on the foundation of confirming genetic relations. 54 monokaryons from “zhong nong 1” and 41 monokaryons from “zhong nong chi bao” were obtained by single spore separation technology.Two abnormal single spore isolates were hybridized and got 150 hybrid strain according the existence of clamp-connection. The fusants were identified by antagonism, esterase isozyme, primordium induction, fruiting test and other methods, the results showed that:(1) 5 Pleurotus eryngii var. tuoliensis were divided into two great categories by antagonism, esterase isozyme and producing mushroom demonstration, one was taken “zhong nong 1”as the representative strain; the other was taken “zhong nong chi bao”as the representative strain.(2) Among 150 hybrid combination strains, 99 strains growed faster were with clam-connection, 75 strains had obvious antagonistic streaks with two parents. equip with the typical esyerase isozyme belt of two parent strains by microscopic examination, antagonism, esterase isozyme and fruiting test demonstration. They were confirmed as hybrid strains, of which 50 hybrids could fruit. The fruiting test showed that 3 strains were excellent fusion hybrid.