Construction Of Triticum Aestivum-Haynaldia Villosa Chromosomes Structural Aberrance Library

Haynaldia villosa L. (2n=14, genome W) is a wild relative of common wheat (Triticum aestivum L.). It is resistant to multiple wheat diseases, such as powdery mildew, wheat yellow mosaic virus, eyespot, take-all, and rusts. It is also credited with good tillering ability, high grain protein content, and tolerance to frost and drought. They are useful potential genetic resources for wheat improvement. Therefore, developing wheat translocation lines with small H. villosa chromosome fragments, preferably interstitial types, can facilitate transfer and utilize genes of interest from this species in wheat improvement. The current study started off with crossing of Co-y rays irradiated T. durum-H.villosa amphiploid with Chinese Spring. Subsequently both homozygous and heterozygous recombinants were selected using genomic in situ hybridization and molecular markers-based techniques. Chromosome structural aberrance with different sizes of H. villosa chromosome fragments were identified to construct the "T. arstivum-H.villosa chromosome structural aberrance library.Through the comparative genomics, Primer 3.0 software was used to design primers from wheat homoeologous group 1 and 3 according to EST sequences. A total of 44 and 63 pairs of 1VS and 3VL primers, respectively, were designed and used to amplify the genomic DNA of Chinese Spring H.villosa, T.durum-H.villosa amphiploid and T. aestivum-H.villosa disomic addition lines DA1V-DA7V. Among them,5 and 6 new primers pairs could amplify specific bands for chromosome 1V and 3 V, respectively.Combining molecular markers and genomic in situ hybridization analysis, this study indentified 9 new T.arstivum-H.villosa chromosome structural aberrance and 27 homezygous. This and previous studies have identified a total of 76 chromosomal structural changes,42 of which are homozygous. Of the 76,13 mapped to chromosome IV,11 to 2 V, 5 to 3V,13 to 4V,11 to 5V,13to 6V, and 10 to chromosome 7V. In the same order,5,8,3, 5,6,9,6 of 1V to 7V are homozygous, respectively. These materials with different chromosomes and different fragments from H.villosa have expanded the T. arstivum-H.villosa chromosomal aberrance library.