Study On The Quantitative Changes Of Mucosal Immunity-associated Cells In Small Intestine Of The One-day Old Yak

Bos Grunniens is Tibetan Plateau endemic species, which feed on alpine pasture. Due to the harsh living conditions of alpine cold and hypoxia, diarrheal diseases occurred frequently, especially for 1-day-old yak diarrheal diseases. Intestinal mucosal immune system is the body’s important immune barrier. Intestinal mucosal immune-related cells play an important role in the intestinal mucosal immune responses.To study the quantitative changes of intestinal mucosal immune cells in the one-day old yak. Immunohistochemistry and histological special staining techniques were used to analyze the distribution and the number of intraepithelial lymphocytes, lamina propria lymphocytes, intraepithelial goblet cells and mast cells, also to determine the properties of each bowel mucus secretion. The number of intraepithelial lymphocytes was decreased from the duodenum to the ileum, and the difference was extremely significant(P <0.01). And the number of lamina propria lymphocytes and mast cells were gradually increased from the duodenum to the ileum, and the difference was extremely significant(P <0.01). The number of goblet cells showed a rising trend from the duodenum to the ileum, and the significant difference was observed between duodenum and jejunum(P <0.05), but the difference between these two intestinal segments and ileum was extremely significant(P<0.01). Specially, goblet cells in duodenum secreted neutral mucus, while goblet cells in jejunum and ileum secreted acid mucus or mixed mucus including neutral and acid mucus. And the duodenal gland near the mucosal layer secreted neutral mucus, while it closing to the muscularis secreted acid mucus. Additionally, intestinal glands secreted mixed mucus including neutral and acid mucus.The quantitative changes of intestinal mucosal immune cells in the one-day old yak suggest that the intestinal mucosa is an important part of the natural barriers.