The Immunology Research Of Chickens Infectious Proventriculitis

Chickens infectious proventriculitis is a chronic digestive tract infectious diseases, caused by a variety of reasons, mainly characterized by glandular stomach swelling congestion, glandular stomach nipple bleeding and ulcer. And it is also one of the important disease hazard raising industry in recent years. This research investigated and studied its epidemiology firstly, explored seven different cytokines changes in the process of the chickens infectious proventriculitis, determined cell factor levels of the onset of chicken and healthy chickens from the body immunology, cayyied on the analysis of the data. MTT method was used for 21 ages chicken to isolate the chicken peripheral blood lymphocytes, cultured and proliferation reactivity testing, Explored the effects on the Newcastle disease and bird flu immune in the progress of the chickens infectious proventriculitis, prepared the pathological tissue section, carried on the corresponding pathological histology observation, further understood the pathogenesis of chickens infectious proventriculitis, laid a foundation to the effective prevention and control of transmission for chicken nfectious proventriculitis. 1. The epidemiological investigation of Chickens infectious proventriculitisChickens infectious proventriculitis is one of the main diseases which endanger the poultry industry, the main symptoms of decreased feed intake, slow growth, glandular swelling, glandular mucosa nipple off, bleeding or ulcers and so on. The disease predominantly affected 3-25 d chicken flock, fatality rate up to 30%, longer course of disease, sustainable 10-15 d. The flock of chickens from the 12 different areas had occurred chickens gonad gastritis at the same time, indicating that the disease may be vertical transmission, which was not excluded under the mode of transmission of vaccine contamination. 2. The cytokine levels in the body after infected the chicken infectious proventriculitisEnzyme-linked immunosorbent assay(ELISA) was used for disease group and healthy group to determine the cytokines of the sampling chickens. Found the cytokines content of the IL- 1, IL- 6, IL- 8, TNF- ɑ, SIg A were increased, while the IL- 2, IFN- gamma were decreased, on the most obvious lesion 21 th day, the cytokines of the infected chickens has the most significant changes. The experimental results proved that after the infected of Chickens infectious proventriculitis, the body appeared different degrees of immunosuppression, caused the immune cytokines changes. 3. The effects on the immune response after infected the chicken infectious proventriculitisMTT method was used for 21 ages of the onset of chicken to isolate the chicken peripheral blood lymphocytes, cultured and proliferation reactivity testing, showed that the content of T cells in the disease chickens is higher than the healthy ones, but there is no significant changes between the two groups. Determined the antibody levels of the Newcastle disease and bird flu by HA and HI method. On the 14 th day, the antibody levels of avian influenza H9 in the healthy chickens were obvious higher than the disease ones, no significance in other days, no significant effect on the antibody levels of Newcastle disease. So the chickens infectious proventriculitis has selective inhibition on the vaccine immune reaction of the organism. 4. The pathological histology observation of Chickens infectious proventriculitisThe glandular stomach, heart, liver, spleen, kidneys, and intestine of Chickens infectious proventriculitis were collected for preparation of paraffin sections. By HE staining, observd the histologic changes of the relevant organ. The results showed that the glandular stomach, heart, liver, spleen and other organs have significant hemorrhage in the infected chickens, and a large number of inflammatory cell iinfiltrated, the lamina propria and submucosa appeared edema in different positions. the glandular stomach nipple bleeded, with extravasated blood between gland tubules. The histopathological changes consistented in the clinical glandular stomach, especially the obvious swelling of glandular stomach, nipple bleeding, ulcers and other characteristics.