Polymorphic Confirm Of Willow EST-SSR And Its Application

Polymorphic confirm of 1082 EST-SSR were analyzed by fourteen Salix babylonica and Salix eriocephala varieties. And chosen 13 polymorphism markers constructed a DNA fingerprint database of 57 leading Salicaceae varieties, Analysed intraspecific genetic variability of markers in Salix purpurea.Main conclusion as followed:1.424 Salix babylonica markers and 658 Salix suchowensis markers were analyzed its polymorphic by fourteen Salix babylonica and Salix eriocephala varieties,respectively. 158 S.babylonica and 341 S. suchowensis markers were successful amplificated. he results showed that 158 were scorable in allele detection, including 148 being polymorphic,accounted for 93.67%.For the 158 scorable markers,a total of 731 alleles were obtained and the number of alleles per locus ranged from 1 to 11(mean 4.62), For the 148 polymorphic markers,the average observed heterozygosity(Ho)was 0.7421,the average expected heterozygosity(He) was 0.3939, the average polymorphic information content(PIC)was 0.3563; 341 were scorable in allele detection,including 290 being polymorphic,accounted for 85.04%, for the 341 scorable markers, a total of 1100 alleles were obtained and the number of alleles per locus was in the range of 1 to 11(mean 3.22).For the 290 polymorphic markers,the average observed heterozygosity(Ho),the average expected heterozygosity(He)and the average polymorphic information content(PIC) was 0.4731,0.5032,0.4305, respectively.The study showed that these EST-SSR markers developed by EST resources of S.babylonica and S. suchowensis have a high polymorphic proportion,were usefor for genetic research.2. In order to obtain some Salicaceae varieties genome information,the flow cytometry was used.The flow cytometry results showed that most of the populous of ploidy are diploid,consistent with records.Salix species are major diploid,and hybrids are tetraploid. The flow cytometry is a useful method for ploidy analysis.Further,the author constructed a fingerprint of 57 leading Salicaceae varieties.The results showed that unique DNA profiles were obtained for each of varieties.For the 13 markers, a total of 127 alleles were obtained and the number of alleles per locus was 4.62. SSR100 had the highest identification,and it 26 allele phenotypes were detected.At least five loci were needed to icentify the 57 Salicaceae varieties.The results indicated that markers used in the study were feasible for variety identification.3.Marker variation in 11 Salix purpurea was analyzed by resequencing.The results revealed that size variant alleles of SSR394 was found in different individual,and the variable numbers of CGT repeats was occur,ranged from 5 to 7.The study showed that the variation of microsatellite repeats and point mutations were the major reason for microsatellite variability in Salix purpurea.